xenograft models

[Blood] Silencing of SOX9 decreased cell proliferation, induced G1/S arrest and increased apoptosis of diffuse large B-cell lymphoma cells, both in vitro and in vivo.

[Oncogene] To understand whether transcriptional activity of RING-finger protein 6 (RNF6) underlied its oncogenic effect, the authors performed integrated chromatin immunoprecipitation-sequencing and RNA-sequencing analysis to identify splicing factor 3b subunit 2 (SF3B2) as a potential downstream target of RNF6.

[Molecular Therapy] The function of the only gene overexpressed in both chemoresistant tumors and tumor tissue relative to normal gastric epithelia, FOXC1, was examined in gastric adenocarcinoma cells, mouse xenograft models, and patient-derived organoid systems, focusing on cancer stem-like cell phenotypes, metastasis, and chemoresistance

[Journal of Experimental & Clinical Cancer Research] Patient-derived glioma stem-like cells, glioblastoma (GBM) cell lines, intracranial GBM xenografts, and GBM sections were used to measure mRNA and protein expression and determine the related molecular mechanisms by qRT-PCR, immunoblot, immunoprecipitation, immunofluorescence, OCR, ECAR, live-cell imaging, and immunohistochemistry.

[npj Breast Cancer] In cell lines, BRCA1 loss was associated with stabilized cyclin E1 during the cell cycle, and BRCA1 siRNA led to increased cyclin E1 in association with reduced phospho-cyclin E1 T62.

[Cell Death & Disease] The loss-of-function assays were used to investigate the effects of trafficking protein 3 on proliferation, apoptosis, cell cycle, and migration of lung squamous cell carcinoma cells.