xenograft models

[Biomedicine & Pharmacotherapy] Scientists summarize the impact of PCAT6 in the development of diverse types of cancers, based on the results of functional studies in cell lines, experiments in xenograft models of cancers and expression studies in samples obtained from human subjects.

[Communications Biology] Researchers demonstrated a method for tumor inoculation using self-assembled hydrogels to reliably generate tumors with low variance in growth.

[Cell Reports] Researchers showed that acute myeloid leukemia cells secreted the stem cell growth factor R-spondin 2 (RSPO2) to promote their self-renewal and prevent cell differentiation.

[Molecular Cancer therapeutics] Researchers performed whole exome sequencing on BRAF-V600E mutant melanoma patient tumors refractory to the combination of BRAF/MEK inhibition and identified acquired oncogenic mutations in NRAS and loss of the tumor suppressor gene CDKN2A.

[Gene Therapy] CAR T cells were designed using a ligand binding domain instead of a single chain variable fragments to target stem-like leukemia cells. Thrombopoietin, the natural ligand to the myeloproliferative leukemia protein (MPL) receptor, was used as the antigen binding domain to engage MPL expressed on HSCs and erythropoietic and megakaryocytic acute myeloid leukemias.

[Oncogene] The authors examined the expression of RNF141 in 64 pairs of colorectal cancer and adjacent normal tissues by real-time PCR, Western blot, and immunohistochemical analysis.