Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsThe authors investigated the hypothesis that neural stem cells release and traffic functional mitochondria via extracellular vesicles to restore mitochondrial function in target cells.
[PLoS Biology]
6807162 {6807162:7CFBE7G7} apa 50 1 160996 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-ed397bda14ffc750f000c0e6ba3a6c7b%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%227CFBE7G7%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Peruzzotti-Jametti%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-07%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPeruzzotti-Jametti%2C%20L.%2C%20Bernstock%2C%20J.%20D.%2C%20Willis%2C%20C.%20M.%2C%20Manferrari%2C%20G.%2C%20Rogall%2C%20R.%2C%20Fernandez-Vizarra%2C%20E.%2C%20Williamson%2C%20J.%20C.%2C%20Braga%2C%20A.%2C%20Bosch%2C%20A.%20van%20den%2C%20Leonardi%2C%20T.%2C%20Krzak%2C%20G.%2C%20Kittel%2C%20%26%23xC1%3B.%2C%20Beninc%26%23xE1%3B%2C%20C.%2C%20Vicario%2C%20N.%2C%20Tan%2C%20S.%2C%20Bastos%2C%20C.%2C%20Bicci%2C%20I.%2C%20Iraci%2C%20N.%2C%20Smith%2C%20J.%20A.%2C%20%26%23x2026%3B%20Pluchino%2C%20S.%20%282021%29.%20Neural%20stem%20cells%20traffic%20functional%20mitochondria%20via%20extracellular%20vesicles.%20%3Ci%3EPLOS%20Biology%3C%5C%2Fi%3E%2C%20%3Ci%3E19%3C%5C%2Fi%3E%284%29%2C%20e3001166.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pbio.3001166%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1371%5C%2Fjournal.pbio.3001166%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3D7CFBE7G7%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Neural%20stem%20cells%20traffic%20functional%20mitochondria%20via%20extracellular%20vesicles%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luca%22%2C%22lastName%22%3A%22Peruzzotti-Jametti%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joshua%20D.%22%2C%22lastName%22%3A%22Bernstock%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cory%20M.%22%2C%22lastName%22%3A%22Willis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giulia%22%2C%22lastName%22%3A%22Manferrari%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rebecca%22%2C%22lastName%22%3A%22Rogall%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Erika%22%2C%22lastName%22%3A%22Fernandez-Vizarra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22James%20C.%22%2C%22lastName%22%3A%22Williamson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alice%22%2C%22lastName%22%3A%22Braga%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aletta%20van%20den%22%2C%22lastName%22%3A%22Bosch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tommaso%22%2C%22lastName%22%3A%22Leonardi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Grzegorz%22%2C%22lastName%22%3A%22Krzak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22%5Cu00c1gnes%22%2C%22lastName%22%3A%22Kittel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristiane%22%2C%22lastName%22%3A%22Beninc%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nunzio%22%2C%22lastName%22%3A%22Vicario%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sisareuth%22%2C%22lastName%22%3A%22Tan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carlos%22%2C%22lastName%22%3A%22Bastos%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Iacopo%22%2C%22lastName%22%3A%22Bicci%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nunzio%22%2C%22lastName%22%3A%22Iraci%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jayden%20A.%22%2C%22lastName%22%3A%22Smith%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ben%22%2C%22lastName%22%3A%22Peacock%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karin%20H.%22%2C%22lastName%22%3A%22Muller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%20J.%22%2C%22lastName%22%3A%22Lehner%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Edit%20Iren%22%2C%22lastName%22%3A%22Buzas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nuno%22%2C%22lastName%22%3A%22Faria%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Massimo%22%2C%22lastName%22%3A%22Zeviani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christian%22%2C%22lastName%22%3A%22Frezza%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alain%22%2C%22lastName%22%3A%22Brisson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicholas%20J.%22%2C%22lastName%22%3A%22Matheson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carlo%22%2C%22lastName%22%3A%22Viscomi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefano%22%2C%22lastName%22%3A%22Pluchino%22%7D%5D%2C%22abstractNote%22%3A%22Neural%20stem%20cell%20%28NSC%29%20transplantation%20induces%20recovery%20in%20animal%20models%20of%20central%20nervous%20system%20%28CNS%29%20diseases.%20Although%20the%20replacement%20of%20lost%20endogenous%20cells%20was%20originally%20proposed%20as%20the%20primary%20healing%20mechanism%20of%20NSC%20grafts%2C%20it%20is%20now%20clear%20that%20transplanted%20NSCs%20operate%20via%20multiple%20mechanisms%2C%20including%20the%20horizontal%20exchange%20of%20therapeutic%20cargoes%20to%20host%20cells%20via%20extracellular%20vesicles%20%28EVs%29.%20EVs%20are%20membrane%20particles%20trafficking%20nucleic%20acids%2C%20proteins%2C%20metabolites%20and%20metabolic%20enzymes%2C%20lipids%2C%20and%20entire%20organelles.%20However%2C%20the%20function%20and%20the%20contribution%20of%20these%20cargoes%20to%20the%20broad%20therapeutic%20effects%20of%20NSCs%20are%20yet%20to%20be%20fully%20understood.%20Mitochondrial%20dysfunction%20is%20an%20established%20feature%20of%20several%20inflammatory%20and%20degenerative%20CNS%20disorders%2C%20most%20of%20which%20are%20potentially%20treatable%20with%20exogenous%20stem%20cell%20therapeutics.%20Herein%2C%20we%20investigated%20the%20hypothesis%20that%20NSCs%20release%20and%20traffic%20functional%20mitochondria%20via%20EVs%20to%20restore%20mitochondrial%20function%20in%20target%20cells.%20Untargeted%20proteomics%20revealed%20a%20significant%20enrichment%20of%20mitochondrial%20proteins%20spontaneously%20released%20by%20NSCs%20in%20EVs.%20Morphological%20and%20functional%20analyses%20confirmed%20the%20presence%20of%20ultrastructurally%20intact%20mitochondria%20within%20EVs%20with%20conserved%20membrane%20potential%20and%20respiration.%20We%20found%20that%20the%20transfer%20of%20these%20mitochondria%20from%20EVs%20to%20mtDNA-deficient%20L929%20Rho0%20cells%20rescued%20mitochondrial%20function%20and%20increased%20Rho0%20cell%20survival.%20Furthermore%2C%20the%20incorporation%20of%20mitochondria%20from%20EVs%20into%20inflammatory%20mononuclear%20phagocytes%20restored%20normal%20mitochondrial%20dynamics%20and%20cellular%20metabolism%20and%20reduced%20the%20expression%20of%20pro-inflammatory%20markers%20in%20target%20cells.%20When%20transplanted%20in%20an%20animal%20model%20of%20multiple%20sclerosis%2C%20exogenous%20NSCs%20actively%20transferred%20mitochondria%20to%20mononuclear%20phagocytes%20and%20induced%20a%20significant%20amelioration%20of%20clinical%20deficits.%20Our%20data%20provide%20the%20first%20evidence%20that%20NSCs%20deliver%20functional%20mitochondria%20to%20target%20cells%20via%20EVs%2C%20paving%20the%20way%20for%20the%20development%20of%20novel%20%28a%29cellular%20approaches%20aimed%20at%20restoring%20mitochondrial%20dysfunction%20not%20only%20in%20multiple%20sclerosis%2C%20but%20also%20in%20degenerative%20neurological%20diseases.%22%2C%22date%22%3A%22Apr%207%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1371%5C%2Fjournal.pbio.3001166%22%2C%22ISSN%22%3A%221545-7885%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.plos.org%5C%2Fplosbiology%5C%2Farticle%3Fid%3D10.1371%5C%2Fjournal.pbio.3001166%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-08T21%3A15%3A56Z%22%7D%7D%5D%7D
Peruzzotti-Jametti, L., Bernstock, J. D., Willis, C. M., Manferrari, G., Rogall, R., Fernandez-Vizarra, E., Williamson, J. C., Braga, A., Bosch, A. van den, Leonardi, T., Krzak, G., Kittel, Á., Benincá, C., Vicario, N., Tan, S., Bastos, C., Bicci, I., Iraci, N., Smith, J. A., … Pluchino, S. (2021). Neural stem cells traffic functional mitochondria via extracellular vesicles. PLOS Biology, 19(4), e3001166. https://doi.org/10.1371/journal.pbio.3001166 Cite
