Researchers demonstrated that equilibration of mouse islets with small molecules in aqueous solutions could be accelerated from greater than 24 to 6 h by increasing incubation temperature to 37 °C.
[Scientific Reports]
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Dolezalova, N., Gruszczyk, A., Barkan, K., Gamble, J. A., Galvin, S., Moreth, T., O’Holleran, K., Mahbubani, K. T., Higgins, J. A., Gribble, F. M., Reimann, F., Surmacki, J., Andrews, S., Casey, J. J., Pampaloni, F., Murphy, M. P., Ladds, G., Slater, N. K. H., & Saeb-Parsy, K. (2021). Accelerating cryoprotectant diffusion kinetics improves cryopreservation of pancreatic islets. Scientific Reports, 11(1), 10418. https://doi.org/10.1038/s41598-021-89853-6 Cite