Two Enhancers Regulate HoxB Genes Expression during Retinoic Acid-Induced Early Embryonic Stem Cells Differentiation through Long-Range Chromatin Interactions

CRISPR/Cas9-mediated individual or compound deletion of the two enhancers significantly inhibited HoxB gene expression, and transcriptome analysis revealed that retinoic acid-induced early ESCs differentiation was blocked in the enhancer KO cells.