Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsResearchers assessed the influence of culture duration on the relative proteome abundance of HepG2 cells cultured as spheroids, which are routinely used to model aspects of the liver.
[Scientific Reports]
7753456 M4T9F5VV items 1 apa 0 default asc 1 162757 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-b65ec66cf53ad9fc4edbc21021dea497%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22M4T9F5VV%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ellero%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-25%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EEllero%2C%20A.%20A.%2C%20van%20den%20Bout%2C%20I.%2C%20Vlok%2C%20M.%2C%20Cromarty%2C%20A.%20D.%2C%20%26amp%3B%20Hurrell%2C%20T.%20%282021%29.%20Continual%20proteomic%20divergence%20of%20HepG2%20cells%20as%20a%20consequence%20of%20long-term%20spheroid%20culture.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2010917.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-89907-9%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-89907-9%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3DM4T9F5VV%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Continual%20proteomic%20divergence%20of%20HepG2%20cells%20as%20a%20consequence%20of%20long-term%20spheroid%20culture%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrea%20Antonio%22%2C%22lastName%22%3A%22Ellero%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Iman%22%2C%22lastName%22%3A%22van%20den%20Bout%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mar%5Cu00e9%22%2C%22lastName%22%3A%22Vlok%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Allan%20Duncan%22%2C%22lastName%22%3A%22Cromarty%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tracey%22%2C%22lastName%22%3A%22Hurrell%22%7D%5D%2C%22abstractNote%22%3A%22Three-dimensional%20models%20are%20considered%20a%20powerful%20tool%20for%20improving%20the%20concordance%20between%20in%20vitro%20and%20in%20vivo%20phenotypes.%20However%2C%20the%20duration%20of%20spheroid%20culture%20may%20influence%20the%20degree%20of%20correlation%20between%20these%20counterparts.%20When%20using%20immortalised%20cell%20lines%20as%20model%20systems%2C%20the%20assumption%20for%20consistency%20and%20reproducibility%20is%20often%20made%20without%20adequate%20characterization%20or%20validation.%20It%20is%20therefore%20essential%20to%20define%20the%20biology%20of%20each%20spheroid%20model%20by%20investigating%20proteomic%20dynamics%2C%20which%20may%20be%20altered%20relative%20to%20culture%20duration.%20As%20an%20example%2C%20we%20assessed%20the%20influence%20of%20culture%20duration%20on%20the%20relative%20proteome%20abundance%20of%20HepG2%20cells%20cultured%20as%20spheroids%2C%20which%20are%20routinely%20used%20to%20model%20aspects%20of%20the%20liver.%20Quantitative%20proteomic%20profiling%20of%20whole%20cell%20lysates%20labelled%20with%20tandem-mass%20tags%20was%20conducted%20using%20liquid%20chromatography-tandem%20mass%20spectrometry%20%28LC%5Cu2013MS%5C%2FMS%29.%20In%20excess%20of%204800%20proteins%20were%20confidently%20identified%2C%20which%20were%20shared%20across%20three%20consecutive%20time%20points%20over%2028%20days.%20The%20HepG2%20spheroid%20proteome%20was%20divergent%20from%20the%20monolayer%20proteome%20after%2014%20days%20in%20culture%20and%20continued%20to%20change%20over%20the%20successive%20culture%20time%20points.%20Proteins%20representing%20the%20recognised%20core%20hepatic%20proteome%2C%20cell%20junction%2C%20extracellular%20matrix%2C%20and%20cell%20adhesion%20proteins%20were%20found%20to%20be%20continually%20modulated.%22%2C%22date%22%3A%222021-05-25%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-89907-9%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-89907-9%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%22NTMX856R%22%5D%2C%22dateModified%22%3A%222021-05-25T20%3A09%3A24Z%22%7D%7D%5D%7D
Ellero, A. A., van den Bout, I., Vlok, M., Cromarty, A. D., & Hurrell, T. (2021). Continual proteomic divergence of HepG2 cells as a consequence of long-term spheroid culture. Scientific Reports, 11(1), 10917. https://doi.org/10.1038/s41598-021-89907-9 Cite
