A Time-Course Raman Spectroscopic Analysis of Spontaneous In Vitro Microcalcifications in a Breast Cancer Cell Line

Using a previously established model of in vitro mineralization, the MDA-MB-231 human breast cancer cell line was induced using two osteogenic agents, inorganic phosphate and β-glycerophosphate, and direct monitoring of the mineralization process was conducted using Raman micro-spectroscopy.