Probing Tissue Transglutaminase Mediated Vascular Smooth Muscle Cell Aging Using a Novel Transamidation-Deficient Tgm2-C277S Mouse Model

Researchers harnessed CRISPR-Cas9 gene editing technology to introduce a mutation at cysteine 277 in the active site of the mouse Tgm2 gene. In vitro, a remodeling stimulus led to the significant loss of vascular compliance in wild-type mice, but not in the Tgm2-C277S or TG2−/− mice.