Intracellular and Exosomal microRNAome Profiling of Human Vascular Smooth Muscle Cells during Replicative Senescence

Researchers aimed to identify the differential expression of intracellular and exosomal miRNA in human VSMCs (hVSMCs) during replicative senescence by isolating intracellular and exosomal miRNAs from hVSMCs and subsequently subjecting them to whole-genome small RNA next-generation sequencing, bioinformatics analysis and qPCR validation.