Generation of CRISPR–Cas9-Mediated Genetic Knockout Human Intestinal Tissue–Derived Enteroid Lines by Lentivirus Transduction and Single-Cell Cloning

Researchers provided a detailed technical pipeline and protocol for using the CRISPR–Cas9 genome editing system to knock out a gene of interest specifically in Human intestinal tissue–derived enteroids by lentiviral transduction and single-cell cloning.