Investigators explored intratumour heterogeneity and plasticity of cancer cell states in pancreatic dutal adenocarcinoma (PDA) in order to reveal cell state-specific regulators. They analyzed...

[Inflammatory Bowel Diseases] The mRNA and protein expression of oncostatin-M and its receptors, gp130, and several fibrotic and chemotactic factors were studied in mucosal biopsies and isolated human intestinal subepithelial myofibroblasts of patients with inflammatory bowel disease and healthy controls and in a model of human intestinal organoids.

[Nature Communications] Scientists demonstrated the usefulness and necessity of a pooled design to reveal donor iPSC line heterogeneity during macrophage cell differentiation and to model rare WT1 mutation-driven kidney disease with chimeric organoids.

[Cell Stem Cell] Researchers constructed human blood-brain barrier (hBBB) assembloids from brain and blood vessel organoids derived from human pluripotent stem cells. They validated the acquisition of BBB-specific molecular, cellular, transcriptomic, and functional characteristics and uncovered extensive neuro-vascular crosstalk with a spatial pattern within hBBB assembloids.

[Aging and Disease] The authors summarized relevant studies on inflammation mediated by the pannexin1 channel in acute ischemic stroke and discussed the possibility of using brain organoid-on-a-chip technology to screen miRNAs that exclusively targeted the pannexin1 channel to provide new therapeutic measures for targeted regulation of pannexin1 channel to reduce inflammation in acute ischemic stroke.

[FinalSpark (Businesswire)] FinalSpark launched the first-ever online platform enabling global researchers to conduct experiments remotely on biological neurons in vitro. The platform provided around-the-clock access to 16 human brain organoids aimed at developing the world’s first living processor.

[Nature Cancer] Using infusion products of individuals with large B cell lymphoma, the authors integrated functional profiling using timelapse imaging microscopy in nanowell grids with subcellular profiling and single-cell RNA sequencing to identify a signature of multifunctional CD8+ T cells.

[Acta Pharmacologica Sinica] Researchers used vascular smooth muscle-specific leucine-rich repeat-containing family 8 A (LRRC8A) knockout mice and an angiotensin II-induced hypertension model.

[Cell] The authors revealed a direct differentiation pathway from HSCs into platelets that is progressively propagated upon aging. The aging-enriched platelet path was decoupled from all other hematopoietic lineages, including erythropoiesis, and operated as an additional layer in parallel with canonical platelet production.

[Cell Stem Cell] Scientists demonstrated that autophagy was an adaptive cytoprotective response to chronic inflammation in the aging murine bone marrow niche.

[Molecular Therapy] Scientists evaluated carbon monoxide (CO)-induced autophagy in human(h) MSCs, and its impact on cell function in murine cecal ligation and puncture.

[Cell Reports] Researchers described the functions of linc-adipose anti-inflammatory (ADAIN), an adipose lincRNA that was downregulated in white adipose tissue of obese humans.