Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsScientists use dCRISPR/Cas9 gene editing with dual gRNAs to introduce large inactivating deletions in the PIM3 gene and achieve stable PIM3 knockout in the human hepatoblastoma cell line, HuH6.
[Cancer Gene Therapy]
6445212 {6445212:ZV6DTC4M} apa 50 1 161280 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-e3bfff5874dd4bb63272d370ba0cb5db%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22ZV6DTC4M%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Marayati%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-16%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMarayati%2C%20R.%2C%20Stafman%2C%20L.%20L.%2C%20Williams%2C%20A.%20P.%2C%20Bownes%2C%20L.%20V.%2C%20Quinn%2C%20C.%20H.%2C%20Markert%2C%20H.%20R.%2C%20Easlick%2C%20J.%20L.%2C%20Stewart%2C%20J.%20E.%2C%20Crossman%2C%20D.%20K.%2C%20Mroczek-Musulman%2C%20E.%2C%20%26amp%3B%20Beierle%2C%20E.%20A.%20%282021%29.%20CRISPR%5C%2FCas9-mediated%20knockout%20of%20PIM3%20suppresses%20tumorigenesis%20and%20cancer%20cell%20stemness%20in%20human%20hepatoblastoma%20cells.%20%3Ci%3ECancer%20Gene%20Therapy%3C%5C%2Fi%3E%2C%201%26%23x2013%3B15.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41417-021-00334-4%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41417-021-00334-4%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DZV6DTC4M%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22CRISPR%5C%2FCas9-mediated%20knockout%20of%20PIM3%20suppresses%20tumorigenesis%20and%20cancer%20cell%20stemness%20in%20human%20hepatoblastoma%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Raoud%22%2C%22lastName%22%3A%22Marayati%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%20L.%22%2C%22lastName%22%3A%22Stafman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adele%20P.%22%2C%22lastName%22%3A%22Williams%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%20V.%22%2C%22lastName%22%3A%22Bownes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Colin%20H.%22%2C%22lastName%22%3A%22Quinn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hooper%20R.%22%2C%22lastName%22%3A%22Markert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Juliet%20L.%22%2C%22lastName%22%3A%22Easlick%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jerry%20E.%22%2C%22lastName%22%3A%22Stewart%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%20K.%22%2C%22lastName%22%3A%22Crossman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elizabeth%22%2C%22lastName%22%3A%22Mroczek-Musulman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elizabeth%20A.%22%2C%22lastName%22%3A%22Beierle%22%7D%5D%2C%22abstractNote%22%3A%22Hepatoblastoma%20remains%20one%20of%20the%20most%20difficult%20childhood%20tumors%20to%20treat%20and%20is%20alarmingly%20understudied.%20We%20previously%20demonstrated%20that%20Proviral%20Insertion%20site%20in%20Maloney%20murine%20leukemia%20virus%20%28PIM%29%20kinases%2C%20specifically%20PIM3%2C%20are%20overexpressed%20in%20human%20hepatoblastoma%20cells%20and%20function%20to%20promote%20tumorigenesis.%20We%20aimed%20to%20use%20CRISPR%5C%2FCas9%20gene%20editing%20with%20dual%20gRNAs%20to%20introduce%20large%20inactivating%20deletions%20in%20the%20PIM3%20gene%20and%20achieve%20stable%20PIM3%20knockout%20in%20the%20human%20hepatoblastoma%20cell%20line%2C%20HuH6.%20PIM3%20knockout%20of%20hepatoblastoma%20cells%20led%20to%20significantly%20decreased%20proliferation%2C%20viability%2C%20and%20motility%2C%20inhibited%20cell-cycle%20progression%2C%20decreased%20tumor%20growth%20in%20a%20xenograft%20murine%20model%2C%20and%20increased%20animal%20survival.%20Analysis%20of%20RNA%20sequencing%20data%20revealed%20that%20PIM3%20knockout%20downregulated%20expression%20of%20pro-migratory%20and%20pro-invasive%20genes%20and%20upregulated%20expression%20of%20genes%20involved%20in%20apoptosis%20and%20differentiation.%20Furthermore%2C%20PIM3%20knockout%20decreased%20hepatoblastoma%20cancer%20cell%20stemness%20as%20evidenced%20by%20decreased%20tumorsphere%20formation%2C%20decreased%20mRNA%20abundance%20of%20stemness%20markers%2C%20and%20decreased%20cell%20surface%20expression%20of%20CD133%2C%20a%20marker%20of%20hepatoblastoma%20stem%20cell-like%20cancer%20cells.%20Reintroduction%20of%20PIM3%20into%20PIM3%20knockout%20cells%20rescued%20the%20malignant%20phenotype.%20Successful%20CRISPR%5C%2FCas9%20knockout%20of%20PIM3%20kinase%20in%20human%20hepatoblastoma%20cells%20confirmed%20the%20role%20of%20PIM3%20in%20promoting%20hepatoblastoma%20tumorigenesis%20and%20cancer%20cell%20stemness.%22%2C%22date%22%3A%222021-04-16%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41417-021-00334-4%22%2C%22ISSN%22%3A%221476-5500%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41417-021-00334-4%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-16T16%3A40%3A10Z%22%7D%7D%5D%7D
Marayati, R., Stafman, L. L., Williams, A. P., Bownes, L. V., Quinn, C. H., Markert, H. R., Easlick, J. L., Stewart, J. E., Crossman, D. K., Mroczek-Musulman, E., & Beierle, E. A. (2021). CRISPR/Cas9-mediated knockout of PIM3 suppresses tumorigenesis and cancer cell stemness in human hepatoblastoma cells. Cancer Gene Therapy, 1–15. https://doi.org/10.1038/s41417-021-00334-4 Cite
