Coculture of Primary Human Colon Monolayer with Human Gut Bacteria

The authors described a protocol that could be used to coculture a primary human colon monolayer with aerotolerant bacteria. Primary human colon cells maintained as organoids were dispersed into single-cell suspensions and then seeded on collagen-coated Transwell inserts, where they attached and proliferated to form confluent monolayers within days of seeding.