Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsInvestigators described a novel in situ cryopreservation technology on the superhydrophobic microwell array chip-chip to preserve the viability of the organoids for future drug sensitivity tests.
[Micromachines]
6445212 6FFLT2WB items 1 apa 0 default asc 1 163038 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-37b683d163a53475b8f3b45a388cbacd%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%226FFLT2WB%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Liu%20et%20al.%22%2C%22parsedDate%22%3A%222021-06%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ELiu%2C%20Q.%2C%20Zhao%2C%20T.%2C%20Wang%2C%20X.%2C%20Chen%2C%20Z.%2C%20Hu%2C%20Y.%2C%20%26amp%3B%20Chen%2C%20X.%20%282021%29.%20In%20Situ%20Vitrification%20of%20Lung%20Cancer%20Organoids%20on%20a%20Microwell%20Array.%20%3Ci%3EMicromachines%3C%5C%2Fi%3E%2C%20%3Ci%3E12%3C%5C%2Fi%3E%286%29%2C%20624.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fmi12060624%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fmi12060624%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3D6FFLT2WB%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22In%20Situ%20Vitrification%20of%20Lung%20Cancer%20Organoids%20on%20a%20Microwell%20Array%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Qiang%22%2C%22lastName%22%3A%22Liu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tian%22%2C%22lastName%22%3A%22Zhao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xianning%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhongyao%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yawei%22%2C%22lastName%22%3A%22Hu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaofang%22%2C%22lastName%22%3A%22Chen%22%7D%5D%2C%22abstractNote%22%3A%22Three-dimensional%20cultured%20patient-derived%20cancer%20organoids%20%28PDOs%29%20represent%20a%20powerful%20tool%20for%20anti-cancer%20drug%20development%20due%20to%20their%20similarity%20to%20the%20in%20vivo%20tumor%20tissues.%20However%2C%20the%20culture%20and%20manipulation%20of%20PDOs%20is%20more%20difficult%20than%202D%20cultured%20cell%20lines%20due%20to%20the%20presence%20of%20the%20culture%20matrix%20and%20the%203D%20feature%20of%20the%20organoids.%20In%20our%20other%20study%2C%20we%20established%20a%20method%20for%20lung%20cancer%20organoid%20%28LCO%29-based%20drug%20sensitivity%20tests%20on%20the%20superhydrophobic%20microwell%20array%20chip%20%28SMAR-chip%29.%20Here%2C%20we%20describe%20a%20novel%20in%20situ%20cryopreservation%20technology%20on%20the%20SMAR-chip%20to%20preserve%20the%20viability%20of%20the%20organoids%20for%20future%20drug%20sensitivity%20tests.%20We%20compared%20two%20cryopreservation%20approaches%20%28slow%20freezing%20and%20vitrification%29%20and%20demonstrated%20that%20vitrification%20performed%20better%20at%20preserving%20the%20viability%20of%20LCOs.%20Next%2C%20we%20developed%20a%20simple%20procedure%20for%20in%20situ%20cryopreservation%20and%20thawing%20of%20the%20LCOs%20on%20the%20SMAR-chip.%20We%20proved%20that%20the%20on-chip%20cryopreserved%20organoids%20can%20be%20recovered%20successfully%20and%2C%20more%20importantly%2C%20showing%20similar%20responses%20to%20anti-cancer%20drugs%20as%20the%20unfrozen%20controls.%20This%20in%20situ%20vitrification%20technology%20eliminated%20the%20harvesting%20and%20centrifugation%20steps%20in%20conventional%20cryopreservation%2C%20making%20the%20whole%20freeze%5Cu2013thaw%20process%20easier%20to%20perform%20and%20the%20preserved%20LCOs%20ready%20to%20be%20used%20for%20the%20subsequent%20drug%20sensitivity%20test.%22%2C%22date%22%3A%222021%5C%2F6%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fmi12060624%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F2072-666X%5C%2F12%5C%2F6%5C%2F624%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-06-03T03%3A47%3A38Z%22%7D%7D%5D%7D
Liu, Q., Zhao, T., Wang, X., Chen, Z., Hu, Y., & Chen, X. (2021). In Situ Vitrification of Lung Cancer Organoids on a Microwell Array. Micromachines, 12(6), 624. https://doi.org/10.3390/mi12060624 Cite
