AKT

[Molecular Therapy-Nucleic Acids] To determine whether skeletal muscle miR-486 was functionally similar in dystrophies and cancer, the authors performed functional limitations and biochemical studies of skeletal muscles of MMTV-Neu mice that mimicked HER2+ breast cancer and MMTV-PyMT mice that mimicked luminal subtype B breast cancer.

[Cell Death Discovery] Researchers performed GEO dataset analysis and identified that claudin1 was a critical regulator of epidermal growth factor receptor tyrosine kinase inhibitors resistance in NSCLC cells.

[Cell Death Discovery] A constitutively-active mutant Akt1 restored Akt-mTOR activation but only partially ameliorated GNE-493-induced prostate cancer cell death. Moreover, GNE-493 was still cytotoxic in Akt1/2-silenced primary prostate cancer cells.

[Frontiers in Molecular Biosciences] In vitro genetic ablation of golgi phosphoprotein 3 (GOLPH3) was performed using small interfering RNA transfection, and a stably overexpressed GOLPH3 colon cancer cell line was constructed using the lentivirus system.

[Molecular Therapy Oncolytics] MicroRNAs (MiRNAs) that targeted ADP-ribosylation factor 6 (ARF6) were predicted and selected in high metastatic breast cancer cells treated with C–C motif chemokine ligand 18 (CCL18).

[Communications Biology] The authors tested the effects of platelet-derived growth factor-BB (PDGF-BB) on primary human brain pericytes in vitro to define pathways related to blood-brain barrier function.