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FACS

Establishment and Characterization of Brain Cancer Primary Cell Cultures From Patients to Enable Phenotypic Screening for New Drugs

[Frontiers in Pharmacology] Enrichment of cancer stem cells was observed in neurospheres by FACS using CD133 antibody and RT-PCR.

Thyroid Hormone Enhances Stem Cell Maintenance and Promotes Lineage-Specific Differentiation in Human Embryonic Stem Cells

[Stem Cell Research & Therapy] To study the impact of triiodothyronine on hPSCs, cell survival and growth were evaluated by measurement of cell growth curve, cloning efficiency, survival after passaging, cell apoptosis, and cell cycle status.

Defined Tumor Antigen-Specific T Cells Potentiate Personalized TCR-T Cell Therapy and Prediction of Immunotherapy Response

[Cell Research] T cells engineered with T cell receptors (TCRs) from naturally occurring tumor antigen-specific T cells in a patient will target personal tumor-specific antigens in their tumor.

Reinvestigation of Classic T Cell Subsets and Identification of Novel Cell Subpopulations by Single-Cell RNA Sequencing

[Journal of Immunology] The authors integrated six bead-enriched T cell subsets with 62,235 single-cell transcriptomes from human PBMCs and clustered them into nine scRNA-seq clustered populations.

Blockade of Beta-Adrenergic Receptors Reduces Cancer Growth and Enhances the Response to Anti-CTLA4 Therapy by Modulating the Tumor Microenvironment

[Oncogene] Investigators showed that oral administration of propranolol delayed tumor progression of MCA205 fibrosarcoma model and MC38 colon cancer model and increased the survival rate of tumor bearing mice.

Silencing ESRP1 Expression Promotes Caspase-Independent Cell Death via Nuclear Translocation of AIF in Colon Cancer Cells

[Cellular Signalling] Scientists analyzed Clinical Proteomic Tumor Analysis Consortium, colon tissue microarray, and colon cancer cells to evaluate the ESRP1 expression levels in colorectal cancer subtypes.

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