Researchers histologically examined 3- to 6-month-old NSG mice, naïve or engrafted with CD34+ human hemopoietic stem cells, and employed a quantitative immunohistochemical approach to identify human and murine cell compartments, comparing the results with the FACS data.
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Human adipose-derived stromal cells and bone marrow-derived stromal cellswere characterized by FACS analysis and tri-lineage differentiation, followed by an intraindividual comparative proteomic analysis upon osteogenic differentiation.
[Journal of Cellular and Molecular Medicine]
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Dadras, M., May, C., Wagner, J. M., Wallner, C., Becerikli, M., Dittfeld, S., Serschnitzki, B., Schilde, L., Guntermann, A., Sengstock, C., Köller, M., Seybold, D., Geßmann, J., Schildhauer, T. A., Lehnhardt, M., Marcus, K., & Behr, B. (n.d.). Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells. Journal of Cellular and Molecular Medicine, n/a(n/a). https://doi.org/10.1111/jcmm.15797 Cite
Scientists determined whether ESCs were capable of generating lung tissue in Nkx2-1-/- mouse embryos with lung agenesis.
[American Journal of Respiratory and Critical Care Medicine]
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Miao, X., Li, Y., Zheng, C., Wang, L., Jin, C., Chen, L., Mi, S., Zhai, W., Wang, Q., & Cai, J. (2020). A promising iPS-based single-cell cloning strategy revealing signatures of somatic mutations in heterogeneous normal cells. Computational and Structural Biotechnology Journal. https://doi.org/10.1016/j.csbj.2020.08.026 Cite
Infection of patient-derived glioma cells with adenovirus (ADV) increased the formation of tumor spheres. ADV infection upregulated stem cell markers and in turn promoted the capacities of self-renewal and multi-lineage differentiation of the infected tumor spheres.
[Cell Communication and Signaling]
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Researchers tested the ability of adeno-associated viral (AAV) vectors carrying the cDNA for BMP-2 to transduce human mesenchymal stem cells, produce BMP-2, and induce osteogenesis in vitro as compared with lentiviral gene therapy with a two-step transcriptional amplification system lentiviral vector.
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Two T-cell specific promoters were assessed: the distal Lck—and the CD3δ-promoter. Transduced hematopoietic stem cells were FACS sorted by mCherry expression and transferred into sublethally irradiated C57/BL6 mice.
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Bogert, N. V., Furkel, J., Din, S., Braren, I., Eckstein, V., Müller, J. A., Uhlmann, L., Katus, H. A., & Konstandin, M. H. (2020). A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus. Scientific Reports, 10(1), 13740. https://doi.org/10.1038/s41598-020-70793-6 Cite
Researchers investigated whether sulforaphane could enhance the chemotherapeutic effects of cisplatin and 5-fluorouracil against head and neck squamous cell carcinomas–cancer stem cells, and its mechanisms of action.
[British Journal of Cancer]
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Investigators developed a therapeutic single-chain variable-fragment antibody that recognises the cancer stem-like cell/cancer-initiating cell antigen, DNAJB8.
[British Journal of Cancer]
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To culture PSCs on feeder cell layers, the elimination of feeder cells is required for biological or gene analysis and for cell passage. Therefore, a simple and cost-effective cell sorting technology is required. The authors focused on dielectrophoresis phenomena for cell separation because these do not require any fluorescent or magnetic dyes or antibodies.