Human CD34+ Hematopoietic Stem Cell–Engrafted NSG Mice: Morphological and Immunophenotypic Features

Researchers histologically examined 3- to 6-month-old NSG mice, naïve or engrafted with CD34+ human hemopoietic stem cells, and employed a quantitative immunohistochemical approach to identify human and murine cell compartments, comparing the results with the FACS data.
[Veterinary Pathology]
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Comparative Proteomic Analysis of Osteogenic Differentiated Human Adipose Tissue and Bone Marrow-Derived Stromal Cells

Human adipose-derived stromal cells and bone marrow-derived stromal cellswere characterized by FACS analysis and tri-lineage differentiation, followed by an intraindividual comparative proteomic analysis upon osteogenic differentiation.
[Journal of Cellular and Molecular Medicine]
Dadras, M., May, C., Wagner, J. M., Wallner, C., Becerikli, M., Dittfeld, S., Serschnitzki, B., Schilde, L., Guntermann, A., Sengstock, C., Köller, M., Seybold, D., Geßmann, J., Schildhauer, T. A., Lehnhardt, M., Marcus, K., & Behr, B. (n.d.). Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells. Journal of Cellular and Molecular Medicine, n/a(n/a). https://doi.org/10.1111/jcmm.15797 Cite
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In Vivo Generation of Lung and Thyroid Tissues from Embryonic Stem Cells using Blastocyst Complementation

Scientists determined whether ESCs were capable of generating lung tissue in Nkx2-1-/- mouse embryos with lung agenesis.
[American Journal of Respiratory and Critical Care Medicine]
Miao, X., Li, Y., Zheng, C., Wang, L., Jin, C., Chen, L., Mi, S., Zhai, W., Wang, Q., & Cai, J. (2020). A promising iPS-based single-cell cloning strategy revealing signatures of somatic mutations in heterogeneous normal cells. Computational and Structural Biotechnology Journal. https://doi.org/10.1016/j.csbj.2020.08.026 Cite
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Adenovirus Infection Promotes the Formation of Glioma Stem Cells from Glioblastoma Cells through the TLR9/NEAT1/STAT3 Pathway

Infection of patient-derived glioma cells with adenovirus (ADV) increased the formation of tumor spheres. ADV infection upregulated stem cell markers and in turn promoted the capacities of self-renewal and multi-lineage differentiation of the infected tumor spheres.
[Cell Communication and Signaling]
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Limited Potential of AAV-Mediated Gene Therapy in Transducing Human Mesenchymal Stem Cells for Bone Repair Applications

Researchers tested the ability of adeno-associated viral (AAV) vectors carrying the cDNA for BMP-2 to transduce human mesenchymal stem cells, produce BMP-2, and induce osteogenesis in vitro as compared with lentiviral gene therapy with a two-step transcriptional amplification system lentiviral vector.
[Gene Therapy]
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A Novel Approach to Genetic Engineering of T-Cell Subsets by Hematopoietic Stem Cell Infection with a Bicistronic Lentivirus

Two T-cell specific promoters were assessed: the distal Lck—and the CD3δ-promoter. Transduced hematopoietic stem cells were FACS sorted by mCherry expression and transferred into sublethally irradiated C57/BL6 mice.
[Scientific Reports]
Bogert, N. V., Furkel, J., Din, S., Braren, I., Eckstein, V., Müller, J. A., Uhlmann, L., Katus, H. A., & Konstandin, M. H. (2020). A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus. Scientific Reports, 10(1), 13740. https://doi.org/10.1038/s41598-020-70793-6 Cite
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Broccoli Extract Increases Drug-Mediated Cytotoxicity towards Cancer Stem Cells of Head and Neck Squamous Cell Carcinoma

Researchers investigated whether sulforaphane could enhance the chemotherapeutic effects of cisplatin and 5-fluorouracil against head and neck squamous cell carcinomas–cancer stem cells, and its mechanisms of action.
[British Journal of Cancer]
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Development of an Artificial Antibody Specific for HLA/Peptide Complex Derived from Cancer Stem-Like Cell/Cancer-Initiating Cell Antigen DNAJB8

Investigators developed a therapeutic single-chain variable-fragment antibody that recognises the cancer stem-like cell/cancer-initiating cell antigen, DNAJB8.
[British Journal of Cancer]
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Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow

To culture PSCs on feeder cell layers, the elimination of feeder cells is required for biological or gene analysis and for cell passage. Therefore, a simple and cost-effective cell sorting technology is required. The authors focused on dielectrophoresis phenomena for cell separation because these do not require any fluorescent or magnetic dyes or antibodies.
[Micromachines]
Takahashi, Y., & Miyata, S. (2020). Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow. Micromachines, 11(8), 734. https://doi.org/10.3390/mi11080734 Cite
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