embryonic stem cells

[Biomedical Microdevices] Scientists differentiated human ESCs to early cardiac mesodermal cells in microfluidic chambers that had a volume of only 30 nanoliters, using discontinuous medium perfusion. To confirm cell pluripotency, they tracked hESC proliferation and immunostained the cells for pluripotency markers SOX2 and OCT3/4.

[Scientific Reports] Researchers investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic stem cell-derived cardiomyocytes.

[Proceedings of the National Academy of Sciences of the United States of America] Scientists described a multicenter effort to test the generality of this pivotal observation, using independent analyses at two laboratories of patient-derived and newly engineered human neurons with heterozygous NRXN1 deletions.

[Nature Communications] In situ hybridization, immunolabelling, cell tracking and transcriptomic analyses show that these embryoids form the three germ layers through a gastrulation process and that they exhibit a wide range of developmental structures, highly similar to neurula-stage mouse embryos.

[International Journal of Molecular Sciences] Investigators generated a ubiquitin-specific protease 3 gene knockout using the CRISPR/Cas9 system and demonstrated that USP3 acts as a protein stabilizer of Oct4 by deubiquitinating Oct4.

[Pharmaceutics] The authors provide a brief overview of iPSCs and discusses recent advances in the establishment of induced nduced tissue-specific stem cells and their possible applications in regenerative medicine.