Lentivirus-mediated overexpression, as well as antisense oligonucleotide-based silencing, modulated H19 in vitro. In vivo analyses used constitutive H19 knockout mice.
[Journal of the American Society of Nephrology]
6445212 BG9GFSRC items 1 apa default asc 1
Haddad, G., Kölling, M., Wegmann, U. A., Dettling, A., Seeger, H., Schmitt, R., Soerensen-Zender, I., Haller, H., Kistler, A. D., Dueck, A., Engelhardt, S., Thum, T., Mueller, T. F., Wüthrich, R. P., & Lorenzen, J. M. (2021). Renal AAV2-Mediated Overexpression of Long Non-Coding RNA H19 Attenuates Ischemic Acute Kidney Injury Through Sponging of microRNA-30a-5p. Journal of the American Society of Nephrology. https://doi.org/10.1681/ASN.2020060775 Cite
In vitro organoids generated from epithelial cells of mouse or patient with idiopathic pulmonary fibrosis co-cultured with young, aged or retinoic acid-pretreated murine fibroblasts were used to test potential targets.
1254621 78GJ7APK items 1 apa default asc 1
Gokey, J. J., Snowball, J., Green, J., Waltamath, M., Spinney, J. J., Black, K. E., Hariri, L. P., Xu, Y., & Perl, A. K. (2021). Pretreatment of aged mice with retinoic acid supports alveolar regeneration via upregulation of reciprocal PDGFA signalling. Thorax. https://doi.org/10.1136/thoraxjnl-2020-214986 Cite
Scientists assessed the current literature detailing the effects of ω-3 polyunsaturated fatty acids (PUFAs) on epithelial cells. Marine-derived ω-3 PUFAs, eicosapentanoic acid and docosahexanoic acid, as well as plant-derived alpha-linolenic acid, are incorporated into intestinal epithelial cell membranes, prevent changes to epithelial permeability, inhibit the production of pro-inflammatory cytokines and eicosanoids and induce the production of anti-inflammatory eicosanoids and docosanoids.
In mild COVID-19, CD8+ resident-memory and CD4+ T-helper-17 cells underwent active expansion towards the end of the trajectory, and were characterized by good effector functions, while in critical COVID-19 they remain more naïve.
6445218 KW62H4LE items 1 apa default asc 1
Wauters, E., Van Mol, P., Garg, A. D., Jansen, S., Van Herck, Y., Vanderbeke, L., Bassez, A., Boeckx, B., Malengier-Devlies, B., Timmerman, A., Van Brussel, T., Van Buyten, T., Schepers, R., Heylen, E., Dauwe, D., Dooms, C., Gunst, J., Hermans, G., Meersseman, P., … Lambrechts, D. (2021). Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages. Cell Research, 1–19. https://doi.org/10.1038/s41422-020-00455-9 Cite
Scientists found that recombinant EREG, BTC, and NRG1 but not Lgr5 ligand R-Spondin promoted growth and proliferation of Apc double mutant colonic organoids.
6445218 7CD2GEL8 items 1 apa default asc 1
Westendorp, F., Karpus, O. N., Koelink, P. J., Vermeulen, J. L. M., Meisner, S., Koster, J., Büller, N. V. J. A., Wildenberg, M. E., Muncan, V., & van den Brink, G. R. (2021). Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation. Oncogene, 1–16. https://doi.org/10.1038/s41388-020-01633-0 Cite
Flow cytometric analyses indicated that induction of epithelial-mesenchymal transition decreased trastuzumab binding, prior to overt loss of HER2 expression in trastuzumab-emtansine-resistant (TDM1R) cells. Kinome analyses of TDM1R cells indicated increased phosphorylation of ErbB1, ErbB4, and FGFR1.
[npj Breast Cancer]
6630899 3NGYR8CD items 1 apa default asc 1
Akhand, S. S., Chen, H., Purdy, S. C., Liu, Z., Anderson, J. C., Willey, C. D., & Wendt, M. K. (2021). Fibroblast growth factor receptor facilitates recurrence of minimal residual disease following trastuzumab emtansine therapy. Npj Breast Cancer, 7(1), 1–11. https://doi.org/10.1038/s41523-020-00213-5 Cite
Researchers investigated the roles of p53, p16INK4a, and p19ARF in the regulation of self-renewal and proliferation of mammary epithelial cells.
[Stem Cell Reports]
6807162 J86XRDM9 items 1 apa default asc 1
Investigators examined whether vinblastine could remove residual undifferentiated human induced pluripotent stem cells in human induced pluripotent stem cells-derived intestinal epithelial cells (hiPSC-IECs), and attempted to generate hiPSC-IECs applicable to transplantation medicine.
[Molecular Therapy-Methods & Clinical Development]
1254621 WTHN6YRV items 1 apa default asc 1
Ichikawa, M., Negoro, R., Kawai, K., Yamashita, T., Takayama, K., & Mizuguchi, H. (2021). Vinblastine treatment decreases the undifferentiated cell contamination of human iPS cell-derived intestinal epithelial-like cells. Molecular Therapy - Methods & Clinical Development, 0(0). https://doi.org/10.1016/j.omtm.2021.01.005 Cite
Researchers examined the roles of 5-Azacytidine and valproic acid in the dedifferentiation of epithelial cell rests of Malassez into stem-like cells. Additionally, the ability of stem-like cells to differentiate into mesenchymal cells was evaluated.
6445212 MLNKZ5WN items 1 apa default asc 1
Yoshida, K., Uehara, O., Kurashige, Y., Paudel, D., Onishi, A., Neopane, P., Hiraki, D., Morikawa, T., Harada, F., Takai, R., Sato, J., Saitoh, M., & Abiko, Y. (2021). Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents. Scientific Reports, 11(1), 1852. https://doi.org/10.1038/s41598-020-79426-4 Cite
Selective growth of murine prostate epithelium under non‐adherent conditions was used to enrich for stem cells. Expression of runt domain transcription factors, stem cell and prostate marker messenger RNAs was determined by quantitative reverse transcription polymerase chain reaction.
6445212 8V9KVAVU items 1 apa default asc 1
Scientists purified different cochlear cell types from neonatal mice, validated the composition of the different groups with single-cell RNA sequencing, and assessed the various groups’ potential to grow into inner ear organoids.