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flow cytometry
Human Immunology News
Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy
[Oxidative Medicine and Cellular Longevity] Researchers investigated the role of flagellin in promoting immunotherapy efficacy through modulating oxidative stress in regulatory B cells.
Dermal Cell News
Effects of Acanthus ebracteatus Vahl. Extract and Verbascoside on Human Dermal Papilla and Murine Macrophage
[Scientific Reports] In controlled in vitro assays it was found that Acanthus ebracteatus Vahl. extract and verbascoside at various concentrations induced dermal papilla cell proliferation which was indicated by an increase in the number of cells in the S and G2/M phases of the cell cycle.
Mesenchymal Cell News
Disruption of Hematopoiesis Attenuates the Osteogenic Differentiation Capacity of Bone Marrow Stromal Cells
[Stem Cell Research & Therapy] The authors investigated the effect of hematopoietic cells on MSCs using various methods, including flow cytometry, adipogenic and osteogenic differentiation analysis, quantitative PCR, western bloting, and microCT analysis.
ESC & iPSC News
iMyoblasts for Ex Vivo and In Vivo Investigations of Human Myogenesis and Disease Modeling
[eLife] Skeletal muscle myoblasts (iMyoblasts) were generated from human iiPSCs using an efficient and reliable transgene-free induction and stem cell selection protocol.
Cancer Stem Cell News
Tanshinone IIA Attenuates the Stemness of Breast Cancer Cells via Targeting the miR-125b/STARD13 Axis
[Experimental Hematology & Oncology] In vitro mammary spheroid formation, flow cytometry assay on CD24−/CD44+ sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells.
Muscle Cell News
The MFF-SIRT1/3 Axis, Regulated by miR-340-5p, Restores Mitochondrial Homeostasis of Hypoxia-Induced Pulmonary Artery Smooth Muscle Cells
[Laboratory Investigation] Pulmonary artery smooth muscle cells were cultured in hypoxia condition for constructing a pulmonary arterial hypertension model in vitro. The expression of genes and proteins were determined by qRT-PCR and western bolt assays.