flow cytometry

[International Immunopharmacology] The authors revealed that sialic acid removal and loading with CSC antigens induced significant molecular, morphological, and functional changes in dendritic cells (DCs) and that this new DC identity may be considered for future combined immunotherapy strategies against breast tumors.

[Cellular & Molecular Biology Letters] The authors suggested that miR-5195-3p functioned as a tumor suppressor by targeting Cyclin L1 (CCNL1) in prostate cancer.

[Cell Cycle] A biotin-microRNA pull-down assay was performed to identify potential miRNAs. qRT-PCR was used to verify the KRT18 and miR-186-3p levels.

[Journal of Hepatology] Scientists identified a proinflammatory hepatocyte subpopulation that developed primarily from periportal hepatocytes and to a lesser extent from pericentral hepatocytes and played key immunoregulatory roles in endotoxemia.

[Cell Death Discovery] Scientists analyzed the regulatory relationship of hypoxia and PVT1 and the mechanism of PVT1 in the hypoxia-induced chemoresistance process of lung cancer.

[Inflammatory Bowel Diseases] Human intestinal organoids expressed core mitochondrial and ECM genes and enriched biologic functions implicated in Crohn’s disease ileal strictures; ECM gene expression was suppressed by both butyrate and eicosatetraynoic acid, with butyrate also suppressing genes regulating epithelial proliferation.