intestinal stem cells

[Stem Cell Reports] Combined bulk transcriptomic and single-cell RNA-sequencing analysis revealed decreased gene expression of markers for differentiated cells in patient-derived organoids derived from metastatic lesions.

[Cell Communication and Signaling] Scientists summarize the signaling pathways contributing to intestinal cell fates and homeostasis functions, focusing on intestinal stem cell proliferation, cell junction formation, remodeling, hypoxia, the impact of intestinal microbiota, the immune system, inflammation, and metabolism.

[Nature] To determine the physiological role of MAP3K2-regulated intestinal stromal cells (MRISCs), scientists co-cultured MRISCs with colonic organoids and found that organoid growth was greater than when co-cultured with MRISC-depleted stromal cells

[Communications Biology] In colitis crypts, a notable cytokine–cytokine receptor, miR-494-3p-targeted EDA2R and the ligand EDA-A2, suppressed colonic stemness and epithelial repair by inhibiting β-catenin/c-Myc. In differentiated intestinal epithelial cells, miR-494-3p inhibited macrophage recruitment, M1 activation and EDA-A2 secretion by targeting IKKβ/NF-κB in colitis.

[Nature Reviews Gastroenterology & Hepatology] Studies on the role of farnesoid X receptor in metabolic diseases and gastrointestinal and liver cancer are discussed, and the potential for development of targeted drugs are summarized.

[mSphere] Human intestinal stem cell-derived enteroids did not support virus replication from every HuNoV-positive stool sample, which led the authors to test and optimize new medium conditions, identify characteristics of stool samples that allow replication, and evaluate consistency of replication over time.