liquid chromatography

[Journal of Proteomics] The authors investigated the protein profile of porcine rotavirus (PoRV)-infected IPEC-J2 cells using quantitative proteomics and provided valuable information to better understand the mechanisms underlying the host responses to PoRV infection in piglets.

[Scientific Reports] Scientists investigated the chemical components of the F. dubia sap extracted by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry and its potential use in cosmetics in terms of cellular antioxidation on keratinocytes, phototoxicity, and irritation on 3D skin cell models following standard tests suggested by the Organization for Economic Cooperation and Development.

[Cell Reports] By performing both whole-genome CRISPR and metabolic inhibitor screens, researchers identified that nicotinamide phosphoribosyltransferase (NAMPT) was required for T cell activation.

[Oncogene] The authors examined the expression of RNF141 in 64 pairs of colorectal cancer and adjacent normal tissues by real-time PCR, Western blot, and immunohistochemical analysis.

[Scientific Reports] Primary human airway epithelial cells, cultured at air–liquid interface, were obtained by clinical airway brushings or from explanted lungs. Human airway Hb mRNA data were from publically available databases; or from RT-PCR.

[Molecular Psychiatry] Investigators utilized human induced pluripotent stem cells to generate reproducible organoids that recapitulate dorsal forebrain development including early corticogenesis.