reporter assay

[Molecular Medicine Reports] Scientists investigated the specific mechanism underlying the effects of miR‑532‑5p on diabetes. Cell viability was determined using an MTT assay. The expression levels of miR‑532‑5P, cyclin D1, Insulin1 and Insulin2 were detected using reverse transcription‑quantitative PCR.

[Journal of Translational Medicine] In vivo and in vitro metastasis assays confirmed miR-4721 promoted cell migration and invasion. Tumor spheroid formation assay, side population assay, and ALDEFLUOR assay verified miR-4721 regulated cancer stem cell-like properties.

[Breast Cancer Research] By knocking down the expression of TAZ in TNBC cells, researchers found that TAZ is essential for the maintenance of telomeres in TNBC cells and that loss of TAZ caused senescence phenotype of TNBC cells.

[Molecular Medicine Reports] Researchers investigated the detailed role of nuclear paraspeckle assembly transcript 1 (NEAT1) in pulmonary arterial hypertension (PAH). Using RT‑qPCR, the expression levels of NEAT1, microRNA (miR)‑34a‑5p, and Krüppel‑like factor 4 were detected in both hypoxia‑treated pulmonary arterial smooth muscle cells and serum from PAH patients.

[International Immunopharmacology] Ectopic expression and knockdown experiments were conducted in pancreatic cancer (PC) cells, PC cells-derived EVs, or mouse xenograft model of PC.

[Journal of Experimental & Clinical Cancer Research] Scientists investigated the role of cold-inducible RNA binding protein (CIRP) in the progression of non-small cell lung cancer (NSCLC) using patient tissue samples, cultured cell lines and animal lung cancer models.