Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsThe biological functions of D. rotundifolia were explored in vitro following the treatment of bronchial epithelial cells, which are the potential targets of the pharmacological effects of the herbal medicine.
[Scientific Reports]
1254621 {1254621:XHVBFKLC} apa 50 1 158409 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-11d8e8ed87cf5a18f8517f7668858cc8%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22XHVBFKLC%22%2C%22library%22%3A%7B%22id%22%3A1254621%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Arruda-Silva%20et%20al.%22%2C%22parsedDate%22%3A%222021-01-27%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EArruda-Silva%2C%20F.%2C%20Bellavite%2C%20P.%2C%20%26amp%3B%20Marzotto%2C%20M.%20%282021%29.%20Low-dose%20Drosera%20rotundifolia%20induces%20gene%20expression%20changes%20in%2016HBE%20human%20bronchial%20epithelial%20cells.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%202356.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-81843-y%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-81843-y%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D1254621%26amp%3Bitem_key%3DXHVBFKLC%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Low-dose%20Drosera%20rotundifolia%20induces%20gene%20expression%20changes%20in%2016HBE%20human%20bronchial%20epithelial%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fabio%22%2C%22lastName%22%3A%22Arruda-Silva%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paolo%22%2C%22lastName%22%3A%22Bellavite%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marta%22%2C%22lastName%22%3A%22Marzotto%22%7D%5D%2C%22abstractNote%22%3A%22Drosera%20rotundifolia%20has%20been%20traditionally%20used%20for%20the%20treatment%20of%20respiratory%20diseases%20in%20phytotherapy%20and%20homeopathy.%20The%20mechanisms%20of%20action%20recognized%20so%20far%20are%20linked%20to%20the%20known%20effects%20of%20specific%20components%2C%20such%20as%20flavonoids%2C%20but%20are%20not%20completely%20understood.%20In%20this%20study%2C%20the%20biological%20functions%20of%20D.%20rotundifolia%20were%20explored%20in%20vitro%20following%20the%20treatment%20of%20bronchial%20epithelial%20cells%2C%20which%20are%20the%20potential%20targets%20of%20the%20pharmacological%20effects%20of%20the%20herbal%20medicine.%20To%20do%20so%2C%20the%20whole%20plant%20ethanolic%20extract%20was%201000-fold%20diluted%20in%20water%20%28D.%20rotundifolia%203%5Cu00d7%29%20and%20added%20to%20a%2016HBE%20human%20cell%20line%20culture%20for%203%5Cu00a0h%20or%206%5Cu00a0h.%20The%20effects%20on%20gene%20expression%20of%20the%20treatments%20and%20corresponding%20controls%20were%20then%20investigated%20by%20RNA%20sequencing.%20The%20differentially%20expressed%20genes%20were%20validated%20through%20RT-qPCR%2C%20and%20the%20enriched%20biological%20functions%20involved%20in%20the%20effects%20of%20treatment%20were%20investigated.%20D.%20rotundifolia%203%5Cu00d7%5Cu2009did%20not%20impair%20cell%20viability%20and%20was%20shown%20to%20be%20a%20stimulant%20of%20cell%20functions%20by%20regulating%20the%20expression%20of%20dozens%20of%20genes%20after%203%5Cu00a0h%2C%20and%20the%20effects%20were%20amplified%20after%206%5Cu00a0h%20of%20treatment.%20The%20main%20differentially%20expressed%20genes%20encoded%20ligands%20of%20epithelial%20growth%20factor%20receptor%2C%20proteins%20involved%20in%20xenobiotic%20detoxification%20and%20cytokines%2C%20suggesting%20that%20D.%20rotundifolia%203%5Cu00d7%5Cu2009could%20stimulate%20self-repair%20systems%2C%20which%20are%20impaired%20in%20airway%20diseases.%20Furthermore%2C%20D.%20rotundifolia%203%5Cu00d7%5Cu2009acts%20on%20a%20complex%20and%20multifaceted%20set%20of%20genes%20and%20may%20potentially%20affect%20different%20layers%20of%20the%20bronchial%20mucosa.%22%2C%22date%22%3A%222021-01-27%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-81843-y%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-81843-y%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-01-27T18%3A37%3A41Z%22%7D%7D%5D%7D
Arruda-Silva, F., Bellavite, P., & Marzotto, M. (2021). Low-dose Drosera rotundifolia induces gene expression changes in 16HBE human bronchial epithelial cells. Scientific Reports, 11(1), 2356. https://doi.org/10.1038/s41598-021-81843-y Cite
