Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsHhuman primary-cultured lymphatic endothelial cells were obtained both from lymphangioleiomyomatosis-affected lung tissues and normal lung tissues using fluorescence-activated cell sorting.
[Scientific Reports]
6445212 8SNXA53D items 1 apa 0 default asc 1 161279 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-9bf6c7307705ee7f0e85a9f973a311a1%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%228SNXA53D%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Nishino%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-16%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENishino%2C%20K.%2C%20Yoshimatsu%2C%20Y.%2C%20Muramatsu%2C%20T.%2C%20Sekimoto%2C%20Y.%2C%20Mitani%2C%20K.%2C%20Kobayashi%2C%20E.%2C%20Okamoto%2C%20S.%2C%20Ebana%2C%20H.%2C%20Okada%2C%20Y.%2C%20Kurihara%2C%20M.%2C%20Suzuki%2C%20K.%2C%20Inazawa%2C%20J.%2C%20Takahashi%2C%20K.%2C%20Watabe%2C%20T.%2C%20%26amp%3B%20Seyama%2C%20K.%20%282021%29.%20Isolation%20and%20characterisation%20of%20lymphatic%20endothelial%20cells%20from%20lung%20tissues%20affected%20by%20lymphangioleiomyomatosis.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%208406.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-88064-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-88064-3%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3D8SNXA53D%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Isolation%20and%20characterisation%20of%20lymphatic%20endothelial%20cells%20from%20lung%20tissues%20affected%20by%20lymphangioleiomyomatosis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Koichi%22%2C%22lastName%22%3A%22Nishino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yasuhiro%22%2C%22lastName%22%3A%22Yoshimatsu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tomoki%22%2C%22lastName%22%3A%22Muramatsu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yasuhito%22%2C%22lastName%22%3A%22Sekimoto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Keiko%22%2C%22lastName%22%3A%22Mitani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Etsuko%22%2C%22lastName%22%3A%22Kobayashi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shouichi%22%2C%22lastName%22%3A%22Okamoto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hiroki%22%2C%22lastName%22%3A%22Ebana%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yoshinori%22%2C%22lastName%22%3A%22Okada%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Masatoshi%22%2C%22lastName%22%3A%22Kurihara%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kenji%22%2C%22lastName%22%3A%22Suzuki%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johji%22%2C%22lastName%22%3A%22Inazawa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kazuhisa%22%2C%22lastName%22%3A%22Takahashi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tetsuro%22%2C%22lastName%22%3A%22Watabe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kuniaki%22%2C%22lastName%22%3A%22Seyama%22%7D%5D%2C%22abstractNote%22%3A%22Lymphangioleiomyomatosis%20%28LAM%29%20is%20a%20rare%20pulmonary%20disease%20characterised%20by%20the%20proliferation%20of%20smooth%20muscle-like%20cells%20%28LAM%20cells%29%2C%20and%20an%20abundance%20of%20lymphatic%20vessels%20in%20LAM%20lesions.%20Studies%20reported%20that%20vascular%20endothelial%20growth%20factor-D%20%28VEGF-D%29%20secreted%20by%20LAM%20cells%20contributes%20to%20LAM-associated%20lymphangiogenesis%2C%20however%2C%20the%20precise%20mechanisms%20of%20lymphangiogenesis%20and%20characteristics%20of%20lymphatic%20endothelial%20cells%20%28LECs%29%20in%20LAM%20lesions%20have%20not%20yet%20been%20elucidated.%20In%20this%20study%2C%20human%20primary-cultured%20LECs%20were%20obtained%20both%20from%20LAM-affected%20lung%20tissues%20%28LAM-LECs%29%20and%20normal%20lung%20tissues%20%28control%20LECs%29%20using%20fluorescence-activated%20cell%20sorting%20%28FACS%29.%20We%20found%20that%20LAM-LECs%20had%20significantly%20higher%20ability%20of%20proliferation%20and%20migration%20compared%20to%20control%20LECs.%20VEGF-D%20significantly%20promoted%20migration%20of%20LECs%20but%20not%20proliferation%20of%20LECs%20in%20vitro.%20cDNA%20microarray%20and%20FACS%20analysis%20revealed%20the%20expression%20of%20vascular%20endothelial%20growth%20factor%20receptor%20%28VEGFR%29-3%20and%20integrin%20%5Cu03b19%20were%20elevated%20in%20LAM-LECs.%20Inhibition%20of%20VEGFR-3%20suppressed%20proliferation%20and%20migration%20of%20LECs%2C%20and%20blockade%20of%20integrin%20%5Cu03b19%20reduced%20VEGF-D-induced%20migration%20of%20LECs.%20Our%20data%20uncovered%20the%20distinct%20features%20of%20LAM-associated%20LECs%2C%20increased%20proliferation%20and%20migration%2C%20which%20may%20be%20due%20to%20higher%20expression%20of%20VEGFR-3%20and%20integrin%20%5Cu03b19.%20Furthermore%2C%20we%20also%20found%20VEGF-D%5C%2FVEGFR-3%20and%20VEGF-D%5C%2F%20integrin%20%5Cu03b19%20signaling%20play%20an%20important%20role%20in%20LAM-associated%20lymphangiogenesis.%22%2C%22date%22%3A%222021-04-16%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-88064-3%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-88064-3%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-16T16%3A35%3A20Z%22%7D%7D%5D%7D
Nishino, K., Yoshimatsu, Y., Muramatsu, T., Sekimoto, Y., Mitani, K., Kobayashi, E., Okamoto, S., Ebana, H., Okada, Y., Kurihara, M., Suzuki, K., Inazawa, J., Takahashi, K., Watabe, T., & Seyama, K. (2021). Isolation and characterisation of lymphatic endothelial cells from lung tissues affected by lymphangioleiomyomatosis. Scientific Reports, 11(1), 8406. https://doi.org/10.1038/s41598-021-88064-3 Cite
