Hypoxia and Heat Stress Affect Epithelial Integrity in a Caco-2/HT-29 Co-Culture

Scientists developed an in vitro intestinal monolayer model using two human colonic epithelial cell lines, Caco-2 and HT-29, co-cultured in Transwell inserts, and investigated the effects of heat treatment and/or hypoxia on the epithelial barrier function.