Dickkopf-Related Protein 3 Alters Aerobic Glycolysis in Pancreatic Cancer BxPC-3 Cells, Promoting CD4⁺ T-cell Activation and Function

The BxPC-3-DKK3 cell line was constructed, and peripheral blood mononuclear cell was prepared. After isolated the CD4⁺ T cells, the lactic acid, glucose uptake ability, cellular viability, proliferation, apoptosis, and markers were detected by PCR and western blot, and the concentrations of multiple cytokines were determined using the ELISA method.