Generation and Characterization of Cardiac Valve Endothelial-Like Cells from Human Pluripotent Stem Cells

The authors described a two-step chemically defined xeno-free method for generating valvular endothelial cell (VEC)-like cells (VELs) from human PSCs (hPSC). When seeded onto the surface of the de-cellularized porcine aortic valve matrix scaffolds, hPSC-derived VELs exhibited superior proliferative and clonogenic potential than the primary VECs and human aortic endothelial cells.