Inducible Caspase-9 Suicide Gene under Control of Endogenous OCT4 to Safeguard Mouse and Human Pluripotent Stem Cell Therapy

Scientists inserted iCasp9 gene into the endogenous OCT4 locus of human and mouse PSCs without affecting their pluripotency. A chemical inducer of dimerization, AP1903, induced iCasp9 activation, which led to the apoptosis of specific undifferentiated PSCs in vitro and in vivo.