Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsMotor function was analyzed by three behavioral tests. Engraftment of human amniotic fluid stem cell in organs were assessed by flow cytometry and RNA scope.
[Scientific Reports]
6445212 DSSSM6C2 items 1 apa 0 default asc 1 161646 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-8d3dac26929ca4db96a437e46c7590e0%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22DSSSM6C2%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Shaw%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-28%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EShaw%2C%20S.%20W.%2C%20Peng%2C%20S.-Y.%2C%20Liang%2C%20C.-C.%2C%20Lin%2C%20T.-Y.%2C%20Cheng%2C%20P.-J.%2C%20Hsieh%2C%20T.-T.%2C%20Chuang%2C%20H.-Y.%2C%20De%20Coppi%2C%20P.%2C%20%26amp%3B%20David%2C%20A.%20L.%20%282021%29.%20Prenatal%20transplantation%20of%20human%20amniotic%20fluid%20stem%20cell%20could%20improve%20clinical%20outcome%20of%20type%20III%20spinal%20muscular%20atrophy%20in%20mice.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%209158.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-88559-z%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-88559-z%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DDSSSM6C2%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Prenatal%20transplantation%20of%20human%20amniotic%20fluid%20stem%20cell%20could%20improve%20clinical%20outcome%20of%20type%20III%20spinal%20muscular%20atrophy%20in%20mice%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Steven%20W.%22%2C%22lastName%22%3A%22Shaw%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shao-Yu%22%2C%22lastName%22%3A%22Peng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ching-Chung%22%2C%22lastName%22%3A%22Liang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tzu-Yi%22%2C%22lastName%22%3A%22Lin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Po-Jen%22%2C%22lastName%22%3A%22Cheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T%5Cu2019sang-T%5Cu2019ang%22%2C%22lastName%22%3A%22Hsieh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hao-Yu%22%2C%22lastName%22%3A%22Chuang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paolo%22%2C%22lastName%22%3A%22De%20Coppi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%20L.%22%2C%22lastName%22%3A%22David%22%7D%5D%2C%22abstractNote%22%3A%22Spinal%20muscular%20atrophy%20%28SMA%29%20is%20a%20single%20gene%20disorder%20affecting%20motor%20function%20in%20uterus.%20Amniotic%20fluid%20is%20an%20alternative%20source%20of%20stem%20cell%20to%20ameliorate%20SMA.%20Therefore%2C%20this%20study%20aims%20to%20examine%20the%20therapeutic%20potential%20of%20Human%20amniotic%20fluid%20stem%20cell%20%28hAFSC%29%20for%20SMA.%20Our%20SMA%20model%20mice%20were%20generated%20by%20deletion%20of%20exon%207%20of%20Smn%20gene%20and%20knock-in%20of%20human%20SMN2.%20A%20total%20of%2016%20SMA%20model%20mice%20were%20injected%20with%201%5Cu2009%5Cu00d7%5Cu2009105%20hAFSC%20in%20uterus%2C%20and%20the%20other%2016%20mice%20served%20as%20the%20negative%20control.%20Motor%20function%20was%20analyzed%20by%20three%20behavioral%20tests.%20Engraftment%20of%20hAFSC%20in%20organs%20were%20assessed%20by%20flow%20cytometry%20and%20RNA%20scope.%20Frequency%20of%20myocytes%2C%20neurons%20and%20innervated%20receptors%20were%20estimated%20by%20staining.%20With%20hAFSC%20transplantation%2C%2015%20fetuses%20survived%20%2893.75%25%20survival%29%20and%20showed%20better%20performance%20in%20all%20motor%20function%20tests.%20Higher%20engraftment%20frequency%20were%20observed%20in%20muscle%20and%20liver.%20Besides%2C%20the%20muscle%20with%20hAFSC%20transplantation%20expressed%20much%20laminin%20%5Cu03b1%20and%20PAX-7.%20Significantly%20higher%20frequency%20of%20myocytes%2C%20neurons%20and%20innervated%20receptors%20were%20observed.%20In%20our%20study%2C%20hAFSC%20engrafted%20on%20neuromuscular%20organs%20and%20improved%20cellular%20and%20behavioral%20outcomes%20of%20SMA%20model%20mice.%20This%20fetal%20therapy%20could%20preserve%20the%20time%20window%20and%20treat%20in%20the%20uterus.%22%2C%22date%22%3A%222021-04-28%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-88559-z%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-88559-z%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-28T17%3A38%3A41Z%22%7D%7D%5D%7D
Shaw, S. W., Peng, S.-Y., Liang, C.-C., Lin, T.-Y., Cheng, P.-J., Hsieh, T.-T., Chuang, H.-Y., De Coppi, P., & David, A. L. (2021). Prenatal transplantation of human amniotic fluid stem cell could improve clinical outcome of type III spinal muscular atrophy in mice. Scientific Reports, 11(1), 9158. https://doi.org/10.1038/s41598-021-88559-z Cite
