ASC

[Biotechnology Journal] Murine adipose-derived MSC line m17.ASC was cultured in agarose-based microwell plates to obtain size-controlled m17.ASC spheroids of an average diameter and cell number of approximately 170 μm and 1100 cells/spheroid, respectively.

[Journal of Experimental Medicine] Scientists showed that a well-established drug target, Bruton’s tyrosine kinase, affected several levels of NLRP3 regulation. BTK directly interacted with NLRP3 in immune cells and phosphorylates four conserved tyrosine residues upon inflammasome activation, in vitro and in vivo.

[Biochimie] To understand the role of the adipose-derived stem cell secretome in breast cancer cell proliferation, death, and phenotype alteration, adipose-derived stem cell-conditioned medium was used to cultivate MCF-7 and MDA-MB-231 cells.

[Stem Cell Reports] Scientists investigated donor-to-donor variability in human gut adult stem cell organoids. They examined intestinal developmental pathways during culture differentiation in ileum- and colon-derived cultures established from multiple donors, showing that differentiation patterns were consistent among cultures.

[Mucosal Immunology] β7 deficiency and MAdCAM-1 blockade specifically depleted antibody secreting cells from the colonic LP, leading to a fecal pan-immunoglobulin deficit, severe colitis, and alterations of microbiota composition.

[Mucosal Immunology] Flow cytometric, ultrastructural and transcriptional profiling showed that αEβ7-expressing antibody-secreting cells represented an undescribed subset of terminally-differentiated intestinal plasma cells that established direct cell to cell contact with intestinal epithelium.