Effect of the Phenylpyrrole Fungicide Fludioxonil on Cell Proliferation and Cardiac Differentiation in Mouse Embryonic Stem Cells

The potential effect of fludioxonil on cardiac differentiation was evaluated in mouse ESCs. Exposure to fludioxonil resulted in reduced size of mouse embryoid bodies and induced increasing expression levels of the pluripotency markers Oct4, Sox2 and Nanog.
[Reproductive Toxicology]
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Direct Reprogramming as a Route to Cardiac Repair

In recent years, direct reprogramming of resident cardiac fibroblasts to induced cardiac-like myocytes has emerged as a promising therapeutic strategy to repurpose the fibrotic response of the injured heart toward a functional myocardium.
[Seminars in Cell & Developmental Biology]
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Patient-Specific iPSC-Derived Cardiomyocytes Reveal Abnormal Regulation of FGF16 in a Familial Atrial Septal Defect

The authors generated a patient-specific iPSC line from a family cohort carrying a hereditary atrial septal defect (ASD) mutation in GATA4 gene, as well as a hESC line carrying the isogenic T280M mutation using the CRISPR/Cas9 genome editing method. The GATA4-mutant iPSCs and ESCs were then differentiated into cardiomyocytes to model GATA4 mutation-associated ASD.
[Cardiovascular Research]
Ye, L., Yu, Y., Zhao, Z.-A., Zhao, D., Ni, X., Wang, Y., Fang, X., Yu, M., Wang, Y., Tang, J.-M., Chen, Y., Shen, Z., Lei, W., & Hu, S. (2021). Patient-specific iPSC-derived cardiomyocytes reveal abnormal regulation of FGF16 in a familial atrial septal defect. Cardiovascular Research, cvab154. https://doi.org/10.1093/cvr/cvab154 Cite
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Injectable Hydrogel for Co-Delivery of 5-Azacytidine in Zein Protein Nanoparticles with Stem Cells for Cardiac Function Restoration

Injectable alginate based microporous hydrogel were used to deliver 5-Azacytidine in zein protein nanoparticle with mesenchymal stem cells for attenuating adverse cardiac remodelling after myocardial infarction.
[International Journal of Pharmaceutics]
Sharma, V., Kumar Dash, S., Manhas, A., Radhakrishnan, J., Jagavelu, K., & Shanker Verma, R. (2021). Injectable Hydrogel for Co-Delivery of 5-Azacytidine in Zein Protein Nanoparticles with Stem Cells for Cardiac Function Restoration. International Journal of Pharmaceutics, 120673. https://doi.org/10.1016/j.ijpharm.2021.120673 Cite
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Interactivity of Biochemical and Physical Stimuli during Epigenetic Conditioning and Cardiomyocytic Differentiation of Stem and Progenitor Cells Derived from Adult Hearts

Mixed populations of cardiosphere-derived stem and progenitor cells containing proliferative and cardiomyogenically committed cells were obtained from adult rat hearts.
[Integrative Biology]
Zeng, W. R., & Doran, P. M. (2021). Interactivity of biochemical and physical stimuli during epigenetic conditioning and cardiomyocytic differentiation of stem and progenitor cells derived from adult hearts. Integrative Biology, zyab003. https://doi.org/10.1093/intbio/zyab003 Cite
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Epithelial Expression of Gata4 and Sox2 Regulates Specification of the Squamous–Columnar Junction via MAPK/ERK Signaling in Mice

By using mouse genetics, lineage tracing, and organoid culture, scientists examined the development of the squamous–columnar junction in the mouse stomach, and thus define the unique features of transitional epithelium.
[Nature Communications]
Sankoda, N., Tanabe, W., Tanaka, A., Shibata, H., Woltjen, K., Chiba, T., Haga, H., Sakai, Y., Mandai, M., Yamamoto, T., Yamada, Y., Uemoto, S., & Kawaguchi, Y. (2021). Epithelial expression of Gata4 and Sox2 regulates specification of the squamous–columnar junction via MAPK/ERK signaling in mice. Nature Communications, 12(1), 560. https://doi.org/10.1038/s41467-021-20906-0 Cite
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ERG1 Plays an Essential Role in Rat Cardiomyocyte Fate Decision by Mediating AKT Signaling

Using the rat embryonic stem cells model, investigators showed that ERG1 was crucial in cardiomyocyte lineage commitment via interactions with integrin β1.
[Stem Cells]
Wang, D., Liu, C., Liu, H., Meng, Y., Lin, F., Gu, Y., Wang, H., Shang, M., Tong, C., Sachinidis, A., Ying, Q., Li, L., & Peng, L. (n.d.). ERG1 Plays an Essential Role in Rat Cardiomyocyte Fate Decision by Mediating AKT Signaling. STEM CELLS, n/a(n/a). https://doi.org/https://doi.org/10.1002/stem.3328 Cite
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The Epigenetic Regulator Mll1 Is Required for Wnt-Driven Intestinal Tumorigenesis and Cancer Stemness

Investigators identified the histone methyltransferase Mll1 as a regulator of Wnt-driven intestinal cancer. Mll1 is highly expressed in Lgr5+ stem cells and human colon carcinomas with increased nuclear β-catenin.
[Nature Communications]
Grinat, J., Heuberger, J., Vidal, R. O., Goveas, N., Kosel, F., Berenguer-Llergo, A., Kranz, A., Wulf-Goldenberg, A., Behrens, D., Melcher, B., Sauer, S., Vieth, M., Batlle, E., Stewart, A. F., & Birchmeier, W. (2020). The epigenetic regulator Mll1 is required for Wnt-driven intestinal tumorigenesis and cancer stemness. Nature Communications, 11(1), 6422. https://doi.org/10.1038/s41467-020-20222-z Cite
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Matrix Stiffness Regulates Myocardial Differentiation of Human Umbilical Cord Mesenchymal Stem Cells

Human umbilical cord-MSCs were cultured on polyacrylamide hydrogels with stiffnesses corresponding to Young’s modulus of 13-16kPa and 62-68kPa which mimic the stiffnesses of healthy heart tissue and fibrotic myocardium.
[Aging]
Aging | Matrix stiffness regulates myocardial differentiation of human umbilical cord mesenchymal stem cells - Full Text. (n.d.). Retrieved December 16, 2020, from https://www.aging-us.com/article/202244/text Cite
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An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development

Researchers describe a fully defined feeder-free protocol to generate early testis-like cells with the ability to be cultured as an organoid, from human induced pluripotent stem cells.
[Stem Cell Reports]
Knarston, I. M., Pachernegg, S., Robevska, G., Ghobrial, I., Er, P. X., Georges, E., Takasato, M., Combes, A. N., Jørgensen, A., Little, M. H., Sinclair, A. H., & Ayers, K. L. (2020). An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2020.10.009 Cite
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BRD4 Interacts with GATA4 to Govern Mitochondrial Homeostasis in Adult Cardiomyocytes

The authors showed that cardiomyocyte-specific deletion of Brd4 in adult mice leads to acute deterioration of cardiac contractile function with mutant animals demonstrating a transcriptomic signature enriched for decreased expression of genes critical for mitochondrial energy production.
[Circulation]
Padmanabhan Arun, Alexanian Michael, Linares-Saldana Ricardo, González-Terán Bárbara, Andreoletti Gaia, Huang Yu, Connolly Andrew J., Kim Wonho, Hsu Austin, Duan Qiming, Winchester Sarah A. B., Felix Franco, Perez-Bermejo Juan A., Wang Qiaohong, Li Li, Shah Parisha P., Haldar Saptarsi M., Jain Rajan, & Srivastava Deepak. (n.d.). BRD4 Interacts with GATA4 to Govern Mitochondrial Homeostasis in Adult Cardiomyocytes. Circulation, 0(0). https://doi.org/10.1161/CIRCULATIONAHA.120.047753 Cite
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