The potential effect of fludioxonil on cardiac differentiation was evaluated in mouse ESCs. Exposure to fludioxonil resulted in reduced size of mouse embryoid bodies and induced increasing expression levels of the pluripotency markers Oct4, Sox2 and Nanog.
7992332 E2K7DG7Y items 1 apa default asc 1
In recent years, direct reprogramming of resident cardiac fibroblasts to induced cardiac-like myocytes has emerged as a promising therapeutic strategy to repurpose the fibrotic response of the injured heart toward a functional myocardium.
[Seminars in Cell & Developmental Biology]
7992332 L7YF54K4 items 1 apa default asc 1
The authors generated a patient-specific iPSC line from a family cohort carrying a hereditary atrial septal defect (ASD) mutation in GATA4 gene, as well as a hESC line carrying the isogenic T280M mutation using the CRISPR/Cas9 genome editing method. The GATA4-mutant iPSCs and ESCs were then differentiated into cardiomyocytes to model GATA4 mutation-associated ASD.
6606345 WESDNY8X items 1 apa default asc 1
Ye, L., Yu, Y., Zhao, Z.-A., Zhao, D., Ni, X., Wang, Y., Fang, X., Yu, M., Wang, Y., Tang, J.-M., Chen, Y., Shen, Z., Lei, W., & Hu, S. (2021). Patient-specific iPSC-derived cardiomyocytes reveal abnormal regulation of FGF16 in a familial atrial septal defect. Cardiovascular Research, cvab154. https://doi.org/10.1093/cvr/cvab154 Cite
Injectable alginate based microporous hydrogel were used to deliver 5-Azacytidine in zein protein nanoparticle with mesenchymal stem cells for attenuating adverse cardiac remodelling after myocardial infarction.
[International Journal of Pharmaceutics]
6445212 ITXXXIWX items 1 apa default asc 1
Sharma, V., Kumar Dash, S., Manhas, A., Radhakrishnan, J., Jagavelu, K., & Shanker Verma, R. (2021). Injectable Hydrogel for Co-Delivery of 5-Azacytidine in Zein Protein Nanoparticles with Stem Cells for Cardiac Function Restoration. International Journal of Pharmaceutics, 120673. https://doi.org/10.1016/j.ijpharm.2021.120673 Cite
Mixed populations of cardiosphere-derived stem and progenitor cells containing proliferative and cardiomyogenically committed cells were obtained from adult rat hearts.
6445218 FRZG6YD3 items 1 apa default asc 1
By using mouse genetics, lineage tracing, and organoid culture, scientists examined the development of the squamous–columnar junction in the mouse stomach, and thus define the unique features of transitional epithelium.
6445218 GI9AZ9I5 items 1 apa default asc 1
Sankoda, N., Tanabe, W., Tanaka, A., Shibata, H., Woltjen, K., Chiba, T., Haga, H., Sakai, Y., Mandai, M., Yamamoto, T., Yamada, Y., Uemoto, S., & Kawaguchi, Y. (2021). Epithelial expression of Gata4 and Sox2 regulates specification of the squamous–columnar junction via MAPK/ERK signaling in mice. Nature Communications, 12(1), 560. https://doi.org/10.1038/s41467-021-20906-0 Cite
Using the rat embryonic stem cells model, investigators showed that ERG1 was crucial in cardiomyocyte lineage commitment via interactions with integrin β1.
1254621 Z5GPP3VX items 1 apa default asc 1
Wang, D., Liu, C., Liu, H., Meng, Y., Lin, F., Gu, Y., Wang, H., Shang, M., Tong, C., Sachinidis, A., Ying, Q., Li, L., & Peng, L. (n.d.). ERG1 Plays an Essential Role in Rat Cardiomyocyte Fate Decision by Mediating AKT Signaling. STEM CELLS, n/a(n/a). https://doi.org/https://doi.org/10.1002/stem.3328 Cite
Investigators identified the histone methyltransferase Mll1 as a regulator of Wnt-driven intestinal cancer. Mll1 is highly expressed in Lgr5+ stem cells and human colon carcinomas with increased nuclear β-catenin.
6445212 NFCTRNQY items 1 apa default asc 1
Grinat, J., Heuberger, J., Vidal, R. O., Goveas, N., Kosel, F., Berenguer-Llergo, A., Kranz, A., Wulf-Goldenberg, A., Behrens, D., Melcher, B., Sauer, S., Vieth, M., Batlle, E., Stewart, A. F., & Birchmeier, W. (2020). The epigenetic regulator Mll1 is required for Wnt-driven intestinal tumorigenesis and cancer stemness. Nature Communications, 11(1), 6422. https://doi.org/10.1038/s41467-020-20222-z Cite
Human umbilical cord-MSCs were cultured on polyacrylamide hydrogels with stiffnesses corresponding to Young’s modulus of 13-16kPa and 62-68kPa which mimic the stiffnesses of healthy heart tissue and fibrotic myocardium.
Researchers describe a fully defined feeder-free protocol to generate early testis-like cells with the ability to be cultured as an organoid, from human induced pluripotent stem cells.
[Stem Cell Reports]
6445218 BETDPQ8D items 1 apa default asc 1
Knarston, I. M., Pachernegg, S., Robevska, G., Ghobrial, I., Er, P. X., Georges, E., Takasato, M., Combes, A. N., Jørgensen, A., Little, M. H., Sinclair, A. H., & Ayers, K. L. (2020). An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2020.10.009 Cite
The authors showed that cardiomyocyte-specific deletion of Brd4 in adult mice leads to acute deterioration of cardiac contractile function with mutant animals demonstrating a transcriptomic signature enriched for decreased expression of genes critical for mitochondrial energy production.
1254621 HQ9VDYVF items 1 apa default asc 1
Padmanabhan Arun, Alexanian Michael, Linares-Saldana Ricardo, González-Terán Bárbara, Andreoletti Gaia, Huang Yu, Connolly Andrew J., Kim Wonho, Hsu Austin, Duan Qiming, Winchester Sarah A. B., Felix Franco, Perez-Bermejo Juan A., Wang Qiaohong, Li Li, Shah Parisha P., Haldar Saptarsi M., Jain Rajan, & Srivastava Deepak. (n.d.). BRD4 Interacts with GATA4 to Govern Mitochondrial Homeostasis in Adult Cardiomyocytes. Circulation, 0(0). https://doi.org/10.1161/CIRCULATIONAHA.120.047753 Cite