flow cytometry

[Chemico-Biological interactions] Scientists synthesized a series of small molecule compounds with the potential to inhibit eIF4E. Among them, the compound EGPI-1 significantly inhibited the proliferation of a variety of lung cancer cells such as A549, NCI-H460, NCI-H1650 and 95D without inhibiting the proliferation of HUVEC cells.

[Hypertension Research] Using multicolor flow cytometry, expression of maturation markers in total dendritic cells (DCs), myeloid DCs, and plasmacytoid DCs and the distribution of peripheral Th17-bearing and T-reg cells were defined.

[Nature Aging] The authors revealed an atlas of age-related changes in the abundance and phenotype of immune cell populations across 12 mouse tissues.

[International Journal of Oral Science] Researchers employed single-cell RNA sequencing to comprehensively study the transcriptional difference between the freshly isolated and monolayer cultured dental-pulp stem cells.

[Neuroscience Letters] Before co-culture, Stem cells from human exfoliated deciduous teeth's (SHED) mitochondria and ESCs's cell membranes were separately labeled with specific fluorescent probes. After co-culture, SHED's mitochondria were tracked by fluorescent microscope and flow cytometry analysis.

[Scientific Reports] Researchers investigated the potential of CSC differentiation into blood cells under chemical hypoxic conditions using CoCl2. CSCs and miPS-LLCcm cells were cultured for one to seven days in the presence of CoCl2, and the expression of VEGFR1/2, Runx1, c-kit, CD31, CD34, and TER-119 was assessed by RT-qPCR, Western blotting and flow cytometry together with Wright-Giemsa staining and immunocytochemistry.