Differentiation of Cancer Stem Cells into Erythroblasts in the Presence of CoCl₂

Researchers investigated the potential of CSC differentiation into blood cells under chemical hypoxic conditions using CoCl₂. CSCs and miPS-LLCcm cells were cultured for one to seven days in the presence of CoCl₂, and the expression of VEGFR1/2, Runx1, c-kit, CD31, CD34, and TER-119 was assessed by RT-qPCR, Western blotting and flow cytometry together with Wright-Giemsa staining and immunocytochemistry.