flow cytometry

[Bone Marrow Transplantation] Investigators reported the intensive bone marrow monitoring of minimal residual disease using flow cytometry and nested reverse transcription polymerase chain reaction (PCR) whenever a fusion transcript allowed it and chimerism by PCR at eleven timepoints in the first two years after transplant.

[Toxicology and Applied Pharmacology] Investigators observed that proportion of CD133+Nanog+ subpopulation in MCF-7 cells was significantly increased after 3,3′-diindolylmethane administration with up-regulated AKT activity by using CyTOF assay.

[Scientific Reports] The authors identified potential anti-inflammatory effects of P2Y12 receptor blockers on monocytes and macrophages.

[Aging-Us] The expression of antisense of KTN1 (KTN1-AS1) in tissues and cells was detected by qRT-PCR, and the relationship between KTN1-AS1 and clinicopathological data of patients with pancreatic cancer was analyzed.

[Cell Death Discovery] Researchers explored the role of exosomal miR-15a derived from mesenchymal stem cells (MSCs) and its related mechanisms in hepatocellular carcinoma (HCC).The exosomes from MSCs restrained HCC cell proliferative, migrating, and invasive potentials, and accelerated their apoptosis.

[Mediators of Inflammation] Peripheral blood endothelial progenitor cells were isolated from nonsmoking non-chronic obstructive pulmonary disease (COPD), smoking non-COPD, and smoking COPD patients. The expressions of p16, p300, and senescence-related genes were detected by RT-PCR and Western Blot.