protein expression

[Molecular Therapy-Nucleic Acids] Investigators explored the relationships among osteoblast differentiation, N-acetyltransferase 10 (NAT10) , and N4-acetylcytidine (ac4C) and found that NAT0 expression and the ac4C level of total RNA were decreased in the bone tissues of OVX mice and osteoporosis patients.

[Scientific Reports] In the ultrasonic microbubble transfection groups, higher pDNA-derived protein expression was found in senescent endothelial progenitor cells (EPCs) than in young EPCs.

[iScience] Scientists showed that AMP kinase (AMPK) activators could induce the reversion of primed mouse epiblast stem cells (mEpiSCs) to the naïve pluripotent state. The addition of AMPK activators alone or together with leukemia inhibitory factor to primed mEpiSCs induced the appearance of naïve-like cells.

[Communications Biology] Researchers applied PRobe-based Imaging for Sequential Multiplexing (PRISM) to facilitate multiplexed imaging with facile, rapid exchange of imaging probes to analyze iPSC-derived cortical and motor neuron cultures that were relevant to psychiatric and neurodegenerative disease models, using over ten protein targets.

[Scientific Reports] Scientists developed an in vitro intestinal monolayer model using two human colonic epithelial cell lines, Caco-2 and HT-29, co-cultured in Transwell inserts, and investigated the effects of heat treatment and/or hypoxia on the epithelial barrier function.

[Journal of Clinical Investigation] Stable ACC1 protein expression suppressed the growth-promoting activity and increased ROS levels with the consumption of NADPH in a primary bone marrow culture, and delayed the onset of acute myeloid leukemia with increases in mature myeloid cells in mouse models.