Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsResearchers assessed miR-675-3p-related regulatory mechanisms in melanoma and the clinical relevance of such regulatory activities.
[Human Cell]
6807162 2UDHS5XM items 1 apa 0 default asc 1 157703 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-36ff6db5b3abfbcf489da012165dfefc%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%222UDHS5XM%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zhao%20et%20al.%22%2C%22parsedDate%22%3A%222021-01-05%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZhao%2C%20C.-C.%2C%20Guo%2C%20H.%2C%20Wang%2C%20Y.%2C%20%26amp%3B%20Li%2C%20J.-H.%20%282021%29.%20Comprehensive%20upstream%20and%20downstream%20regulatory%20analyses%20identify%20miR-675-3p%20as%20a%20potential%20prognostic%20biomarker%20in%20melanoma.%20%3Ci%3EHuman%20Cell%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs13577-020-00473-0%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs13577-020-00473-0%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3D2UDHS5XM%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Comprehensive%20upstream%20and%20downstream%20regulatory%20analyses%20identify%20miR-675-3p%20as%20a%20potential%20prognostic%20biomarker%20in%20melanoma%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cai-Chou%22%2C%22lastName%22%3A%22Zhao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hao%22%2C%22lastName%22%3A%22Guo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ying%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jiu-Hong%22%2C%22lastName%22%3A%22Li%22%7D%5D%2C%22abstractNote%22%3A%22This%20study%20assessed%20miR-675-3p-related%20regulatory%20mechanisms%20in%20melanoma%20and%20the%20clinical%20relevance%20of%20such%20regulatory%20activities.%20We%20downloaded%20miRNA%20mature%20strand%20expression%20RNA-Seq%2C%20phenotypic%2C%20and%20DNA%20methylation%20data%20pertaining%20to%20the%20TCGA%20Melanoma%20cohort.%20Differentially%20expressed%20miRNAs%20%28DEMs%29%20between%20metastatic%20and%20primary%20melanoma%20patient%20tissues%20were%20then%20identified%2C%20and%20miR-675-3p%20expression%20in%20melanoma%20patient%20peripheral%20blood%20was%20confirmed%20using%20the%20GSE20994%20GEO%20dataset%2C%20while%20its%20expression%20in%20melanoma%20cell%20lines%20was%20evaluated%20via%20qRT-RCR.%20The%20clinical%20and%20prognostic%20implications%20of%20miR-675-3p%20in%20melanoma%20were%20assessed%2C%20and%20miR-675-3p%20target%20genes%20were%20identified%20using%20bioinformatics%20tools.%20Functional%20roles%20of%20this%20miRNA%20were%20explored%20via%20Gene%20Ontology%20%28GO%29%20and%20Kyoto%20Encyclopedia%20of%20Genes%20and%20Genomes%20%28KEGG%29%20analyses.%20We%20identified%203%20and%2022%20miRNAs%20that%20were%20up-%20and%20downregulated%2C%20respectively%2C%20in%20metastatic%20melanoma%20samples%20relative%20to%20primary%20melanoma%20samples.%20Upregulation%20of%20miR-675-3p%20was%20associated%20with%20poorer%20overall%20patient%20survival%2C%20tumor%20histologic%20grade%2C%20and%20Clark%27s%20level.%20Consistently%2C%20miR-675-3p%20was%20also%20overexpressed%20in%20the%20peripheral%20blood%20of%20melanoma%20patients%20relative%20to%20healthy%20controls%2C%20and%20in%20melanoma%20cell%20lines%20relative%20to%20control%20cells.%20Gene%20regulatory%20networks%20indicated%20that%2032%20transcription%20factors%20control%20miR-675-3p%20expression%2C%20and%20that%20it%2C%20in%20turn%2C%20regulates%2010%20target%20genes.%20KEGG%20analyses%20indicated%20that%20these%20genes%20were%20associated%20with%20cell%20cycle%2C%20transcriptional%20misregulation%20in%20cancer%2C%20TGF-beta%20signaling%2C%20and%20HIF-1%20signaling%20pathways.%20Gain-of-function%20assays%20revealed%20that%20miR-675-3p%20could%20promote%20cell%20proliferation%20via%20accelerating%20cell%20cycle%20progression.%20Western%20blotting%20results%20indicated%20that%20miR-675-3p%20could%20active%20TGF-beta%20and%20HIF-1%20signaling.%20Through%20upstream%20and%20downstream%20analyses%20of%20miR-675-3p-related%20regulatory%20activity%2C%20we%20confirmed%20that%20this%20miRNA%20participates%20in%20key%20melanoma-related%20processes%20and%20offers%20value%20as%20a%20prognostic%20biomarker%20in%20melanoma%20patients.%22%2C%22date%22%3A%222021-01-05%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1007%5C%2Fs13577-020-00473-0%22%2C%22ISSN%22%3A%221749-0774%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs13577-020-00473-0%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-01-07T23%3A28%3A09Z%22%7D%7D%5D%7D
Zhao, C.-C., Guo, H., Wang, Y., & Li, J.-H. (2021). Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma. Human Cell. https://doi.org/10.1007/s13577-020-00473-0 Cite
