Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsScientists showed that hundreds of intronic polyadenylation sites were differentially used during keratinocyte differentiation, which was accompanied by downregulation of the Cleavage and Polyadenylation Specificity Factor (CPSF) complex.
[Nature Communications]
6807162 {6807162:U9X4ZYET} apa 50 1 158108 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-3d35f2ed16b76b342e70574825e1e4c8%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22U9X4ZYET%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chen%20et%20al.%22%2C%22parsedDate%22%3A%222021-01-19%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EChen%2C%20X.%2C%20Lloyd%2C%20S.%20M.%2C%20Kweon%2C%20J.%2C%20Gamalong%2C%20G.%20M.%2C%20%26amp%3B%20Bao%2C%20X.%20%282021%29.%20Epidermal%20progenitors%20suppress%20GRHL3-mediated%20differentiation%20through%20intronic%20polyadenylation%20promoted%20by%20CPSF-HNRNPA3%20collaboration.%20%3Ci%3ENature%20Communications%3C%5C%2Fi%3E%2C%20%3Ci%3E12%3C%5C%2Fi%3E%281%29%2C%20448.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-020-20674-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41467-020-20674-3%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3DU9X4ZYET%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Epidermal%20progenitors%20suppress%20GRHL3-mediated%20differentiation%20through%20intronic%20polyadenylation%20promoted%20by%20CPSF-HNRNPA3%20collaboration%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xin%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sarah%20M.%22%2C%22lastName%22%3A%22Lloyd%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Junghun%22%2C%22lastName%22%3A%22Kweon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giovanni%20M.%22%2C%22lastName%22%3A%22Gamalong%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaomin%22%2C%22lastName%22%3A%22Bao%22%7D%5D%2C%22abstractNote%22%3A%22In%20self-renewing%20somatic%20tissue%20such%20as%20skin%20epidermis%2C%20terminal%20differentiation%20genes%20must%20be%20suppressed%20in%20progenitors%20to%20sustain%20regenerative%20capacity.%20Here%20we%20show%20that%20hundreds%20of%20intronic%20polyadenylation%20%28IpA%29%20sites%20are%20differentially%20used%20during%20keratinocyte%20differentiation%2C%20which%20is%20accompanied%20by%20downregulation%20of%20the%20Cleavage%20and%20Polyadenylation%20Specificity%20Factor%20%28CPSF%29%20complex.%20Sustained%20CPSF%20expression%20in%20undifferentiated%20keratinocytes%20requires%20the%20contribution%20from%20the%20transcription%20factor%20MYC.%20In%20keratinocytes%20cultured%20in%20undifferentiation%20condition%2C%20CSPF%20knockdown%20induces%20premature%20differentiation%20and%20partially%20affects%20dynamically%20used%20IpA%20sites.%20These%20sites%20include%20an%20IpA%20site%20located%20in%20the%20first%20intron%20of%20the%20differentiation%20activator%20GRHL3.%20CRISPR%20knockout%20of%20GRHL3%20IpA%20increased%20full-length%20GRHL3%20mRNA%20expression.%20Using%20a%20targeted%20genetic%20screen%2C%20we%20identify%20that%20HNRNPA3%20interacts%20with%20CPSF%20and%20enhances%20GRHL3%20IpA.%20Our%20data%20suggest%20a%20model%20where%20the%20interaction%20between%20CPSF%20and%20RNA-binding%20proteins%2C%20such%20as%20HNRNPA3%2C%20promotes%20site-specific%20IpA%20and%20suppresses%20premature%20differentiation%20in%20progenitors.%22%2C%22date%22%3A%222021-01-19%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41467-020-20674-3%22%2C%22ISSN%22%3A%222041-1723%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41467-020-20674-3%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-01-19T19%3A28%3A16Z%22%7D%7D%5D%7D
Chen, X., Lloyd, S. M., Kweon, J., Gamalong, G. M., & Bao, X. (2021). Epidermal progenitors suppress GRHL3-mediated differentiation through intronic polyadenylation promoted by CPSF-HNRNPA3 collaboration. Nature Communications, 12(1), 448. https://doi.org/10.1038/s41467-020-20674-3 Cite
