Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsScientists performed a longitudinal comparison of the transcriptomic profiles of three hiPSC lines that displayed differential myogenic specification, one robust and two blunted.
[Science Advances]
6445218 {6445218:E5HVGDAA} apa 50 1 160264 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-c773e42188cf32ee75e929123277d7b2%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22E5HVGDAA%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Nayak%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-01%22%2C%22numChildren%22%3A3%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENayak%2C%20P.%2C%20Colas%2C%20A.%2C%20Mercola%2C%20M.%2C%20Varghese%2C%20S.%2C%20%26amp%3B%20Subramaniam%2C%20S.%20%282021%29.%20Temporal%20mechanisms%20of%20myogenic%20specification%20in%20human%20induced%20pluripotent%20stem%20cells.%20%3Ci%3EScience%20Advances%3C%5C%2Fi%3E%2C%20%3Ci%3E7%3C%5C%2Fi%3E%2812%29%2C%20eabf7412.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.abf7412%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1126%5C%2Fsciadv.abf7412%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DE5HVGDAA%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Temporal%20mechanisms%20of%20myogenic%20specification%20in%20human%20induced%20pluripotent%20stem%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Nayak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Colas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M.%22%2C%22lastName%22%3A%22Mercola%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Varghese%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Subramaniam%22%7D%5D%2C%22abstractNote%22%3A%22Understanding%20the%20mechanisms%20of%20myogenesis%20in%20human%20induced%20pluripotent%20stem%20cells%20%28hiPSCs%29%20is%20a%20prerequisite%20to%20achieving%20patient-specific%20therapy%20for%20diseases%20of%20skeletal%20muscle.%20hiPSCs%20of%20different%20origin%20show%20distinctive%20kinetics%20and%20ability%20to%20differentiate%20into%20myocytes.%20To%20address%20the%20unique%20cellular%20and%20temporal%20context%20of%20hiPSC%20differentiation%2C%20we%20perform%20a%20longitudinal%20comparison%20of%20the%20transcriptomic%20profiles%20of%20three%20hiPSC%20lines%20that%20display%20differential%20myogenic%20specification%2C%20one%20robust%20and%20two%20blunted.%20We%20detail%20temporal%20differences%20in%20mechanisms%20that%20lead%20to%20robust%20myogenic%20specification.%20We%20show%20gene%20expression%20signatures%20of%20putative%20cell%20subpopulations%20and%20extracellular%20matrix%20components%20that%20may%20support%20myogenesis.%20Furthermore%2C%20we%20show%20that%20targeted%20knockdown%20of%20ZIC3%20at%20the%20outset%20of%20differentiation%20leads%20to%20improved%20myogenic%20specification%20in%20blunted%20hiPSC%20lines.%20Our%20study%20suggests%20that%20%5Cu03b2-catenin%20transcriptional%20cofactors%20mediate%20cross-talk%20between%20multiple%20cellular%20processes%20and%20exogenous%20cues%20to%20facilitate%20specification%20of%20hiPSCs%20to%20mesoderm%20lineage%2C%20leading%20to%20robust%20myogenesis.%5CnMechanisms%20of%20myogenic%20commitment%20are%20deciphered%20through%20omics%20analysis%20of%20hiPSC-specific%20lineage%20specification%20measurements.%5CnMechanisms%20of%20myogenic%20commitment%20are%20deciphered%20through%20omics%20analysis%20of%20hiPSC-specific%20lineage%20specification%20measurements.%22%2C%22date%22%3A%222021%5C%2F03%5C%2F01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1126%5C%2Fsciadv.abf7412%22%2C%22ISSN%22%3A%222375-2548%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fadvances.sciencemag.org%5C%2Fcontent%5C%2F7%5C%2F12%5C%2Feabf7412%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-18T19%3A55%3A42Z%22%7D%7D%5D%7D
Nayak, P., Colas, A., Mercola, M., Varghese, S., & Subramaniam, S. (2021). Temporal mechanisms of myogenic specification in human induced pluripotent stem cells. Science Advances, 7(12), eabf7412. https://doi.org/10.1126/sciadv.abf7412 Cite
