hERG-Deficient Human Embryonic Stem Cell-Derived Cardiomyocytes for Modelling QT Prolongation

0
253
The KCNH2 was knocked out in the hESC H9 line using the CRISPR/Cas9 system. Using a chemically defined differentiation protocol, investigators obtained and verified human ether-a-go-go-related gene(hERG)-deficient cardiomyocytes. Subsequently, high-throughput microelectrode array assays and drug interventions were performed to characterise the electrophysiological signatures of hERG-deficient cell lines.
[Stem Cell Research & Therapy]
6606345 {6606345:4ILB7SVM} 1 apa 50 default 1 162139 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%224ILB7SVM%22%2C%22library%22%3A%7B%22id%22%3A6606345%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chang%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-07%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BChang%2C%20Y.%2C%20Li%2C%20Y.%2C%20Bai%2C%20R.%2C%20Wu%2C%20F.%2C%20Ma%2C%20S.%2C%20Saleem%2C%20A.%2C%20Zhang%2C%20S.%2C%20Jiang%2C%20Y.%2C%20Dong%2C%20T.%2C%20Guo%2C%20T.%2C%20Hang%2C%20C.%2C%20Lu%2C%20W.%2C%20Jiang%2C%20H.%2C%20%26amp%3B%20Lan%2C%20F.%20%282021%29.%20hERG-deficient%20human%20embryonic%20stem%20cell-derived%20cardiomyocytes%20for%20modelling%20QT%20prolongation.%20%26lt%3Bi%26gt%3BStem%20Cell%20Research%20%26amp%3B%20Therapy%26lt%3B%5C%2Fi%26gt%3B%2C%20%26lt%3Bi%26gt%3B12%26lt%3B%5C%2Fi%26gt%3B%281%29%2C%20278.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-ItemURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs13287-021-02346-1%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs13287-021-02346-1%26lt%3B%5C%2Fa%26gt%3B%20%26lt%3Ba%20title%3D%26%23039%3BCite%20in%20RIS%20Format%26%23039%3B%20class%3D%26%23039%3Bzp-CiteRIS%26%23039%3B%20data-zp-cite%3D%26%23039%3Bapi_user_id%3D6606345%26amp%3Bitem_key%3D4ILB7SVM%26%23039%3B%20href%3D%26%23039%3Bjavascript%3Avoid%280%29%3B%26%23039%3B%26gt%3BCite%26lt%3B%5C%2Fa%26gt%3B%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22hERG-deficient%20human%20embryonic%20stem%20cell-derived%20cardiomyocytes%20for%20modelling%20QT%20prolongation%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yun%22%2C%22lastName%22%3A%22Chang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ya-nan%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rui%22%2C%22lastName%22%3A%22Bai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fujian%22%2C%22lastName%22%3A%22Wu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shuhong%22%2C%22lastName%22%3A%22Ma%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amina%22%2C%22lastName%22%3A%22Saleem%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Siyao%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Youxu%22%2C%22lastName%22%3A%22Jiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tao%22%2C%22lastName%22%3A%22Dong%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tianwei%22%2C%22lastName%22%3A%22Guo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chengwen%22%2C%22lastName%22%3A%22Hang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wen-jing%22%2C%22lastName%22%3A%22Lu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hongfeng%22%2C%22lastName%22%3A%22Jiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Feng%22%2C%22lastName%22%3A%22Lan%22%7D%5D%2C%22abstractNote%22%3A%22%5Cu4c6f%5Cu6e67%5Cu2d51%5Cu5420%5Cu7379%5Cu6e64%5Cu726f%5Cu6d65%5Cu2074%5Cu7970%5Cu6520%5Cu3220%5Cu284c%5Cu5154%5Cu3229%5Cu2069%5Cu7320%5Cu6120%5Cu636f%5Cu6d6d%5Cu6f6e%5Cu206d%5Cu616c%5Cu6967%5Cu6e61%5Cu6e74%5Cu2068%5Cu6572%5Cu6564%5Cu6974%5Cu6172%5Cu7920%5Cu6172%5Cu7268%5Cu7974%5Cu686d%5Cu6961%5Cu2e20%5Cu4475%5Cu6520%5Cu746f%5Cu2074%5Cu6865%5Cu206c%5Cu6163%5Cu6b20%5Cu6f66%5Cu2073%5Cu7569%5Cu7461%5Cu626c%5Cu6520%5Cu616e%5Cu696d%5Cu616c%5Cu2061%5Cu6e64%5Cu2068%5Cu756d%5Cu616e%5Cu206d%5Cu6f64%5Cu656c%5Cu732c%5Cu2074%5Cu6865%5Cu2070%5Cu6174%5Cu686f%5Cu6765%5Cu6e65%5Cu7369%5Cu7320%5Cu6f66%5Cu204c%5Cu5154%5Cu3220%5Cu6361%5Cu7573%5Cu6564%5Cu2062%5Cu7920%5Cu6875%5Cu6d61%5Cu6e20%5Cu6574%5Cu6865%5Cu722d%5Cu612d%5Cu676f%5Cu2d67%5Cu6f2d%5Cu7265%5Cu6c61%5Cu7465%5Cu6420%5Cu6765%5Cu6e65%5Cu2028%5Cu6845%5Cu5247%5Cu2920%5Cu6465%5Cu6669%5Cu6369%5Cu656e%5Cu6379%5Cu2069%5Cu7320%5Cu7374%5Cu696c%5Cu6c20%5Cu756e%5Cu636c%5Cu6561%5Cu722e%5Cu2049%5Cu6e20%5Cu7468%5Cu6973%5Cu2073%5Cu7475%5Cu6479%5Cu2c20%5Cu7765%5Cu2067%5Cu656e%5Cu6572%5Cu6174%5Cu6564%5Cu2061%5Cu6e20%5Cu6845%5Cu5247%5Cu2d64%5Cu6566%5Cu6963%5Cu6965%5Cu6e74%5Cu2068%5Cu756d%5Cu616e%5Cu2063%5Cu6172%5Cu6469%5Cu6f6d%5Cu796f%5Cu6379%5Cu7465%5Cu2028%5Cu434d%5Cu2920%5Cu6d6f%5Cu6465%5Cu6c20%5Cu7468%5Cu6174%5Cu2073%5Cu696d%5Cu756c%5Cu6174%5Cu6573%5Cu20e2%5Cu8098%5Cu6875%5Cu6d61%5Cu6e20%5Cu686f%5Cu6d6f%5Cu7a79%5Cu676f%5Cu7573%5Cu2068%5Cu4552%5Cu4720%5Cu6d75%5Cu7461%5Cu7469%5Cu6f6e%5Cu73e2%5Cu8099%5Cu2074%5Cu6f20%5Cu6578%5Cu706c%5Cu6f72%5Cu6520%5Cu7468%5Cu6520%5Cu756e%5Cu6465%5Cu726c%5Cu7969%5Cu6e67%5Cu2069%5Cu6d70%5Cu6163%5Cu7420%5Cu6f66%5Cu2068%5Cu4552%5Cu4720%5Cu6479%5Cu7366%5Cu756e%5Cu6374%5Cu696f%5Cu6e20%5Cu616e%5Cu6420%5Cu7468%5Cu6520%5Cu6765%5Cu6e6f%5Cu7479%5Cu7065%5Cue280%5Cu9370%5Cu6865%5Cu6e6f%5Cu7479%5Cu7065%5Cu2072%5Cu656c%5Cu6174%5Cu696f%5Cu6e73%5Cu6869%5Cu7020%5Cu6f66%5Cu2068%5Cu4552%5Cu4720%5Cu6465%5Cu6669%5Cu6369%5Cu656e%5Cu6379%3F%22%2C%22date%22%3A%22May%207%2C%202021%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1186%5C%2Fs13287-021-02346-1%22%2C%22ISSN%22%3A%221757-6512%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs13287-021-02346-1%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-05-12T18%3A18%3A56Z%22%7D%7D%5D%7D
Chang, Y., Li, Y., Bai, R., Wu, F., Ma, S., Saleem, A., Zhang, S., Jiang, Y., Dong, T., Guo, T., Hang, C., Lu, W., Jiang, H., & Lan, F. (2021). hERG-deficient human embryonic stem cell-derived cardiomyocytes for modelling QT prolongation. Stem Cell Research & Therapy, 12(1), 278. https://doi.org/10.1186/s13287-021-02346-1 Cite
Full Article