hERG-Deficient Human Embryonic Stem Cell-Derived Cardiomyocytes for Modelling QT Prolongation

By
Justin.choi
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The KCNH2 was knocked out in the hESC H9 line using the CRISPR/Cas9 system. Using a chemically defined differentiation protocol, investigators obtained and verified human ether-a-go-go-related gene(hERG)-deficient cardiomyocytes. Subsequently, high-throughput microelectrode array assays and drug interventions were performed to characterise the electrophysiological signatures of hERG-deficient cell lines.
[Stem Cell Research & Therapy]
Chang, Y., Li, Y., Bai, R., Wu, F., Ma, S., Saleem, A., Zhang, S., Jiang, Y., Dong, T., Guo, T., Hang, C., Lu, W., Jiang, H., & Lan, F. (2021). hERG-deficient human embryonic stem cell-derived cardiomyocytes for modelling QT prolongation. Stem Cell Research & Therapy, 12(1), 278. https://doi.org/10.1186/s13287-021-02346-1 Cite
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