Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsIslets were kept intact or dispersed into single cells and cultured in the presence of harmine, glucose, or heparin-binding epidermal growth factor-like growth factor (HB-EGF), and subsequently analyzed by immunohistochemistry or flow cytometry.
[Scientific Reports]
7753456 3Z68GUVE items 1 apa 0 default asc 1 162884 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-0853acd613f9dda70538d96347c4183d%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%223Z68GUVE%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Maachi%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-28%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMaachi%2C%20H.%2C%20Ghislain%2C%20J.%2C%20Tremblay%2C%20C.%2C%20%26amp%3B%20Poitout%2C%20V.%20%282021%29.%20Pronounced%20proliferation%20of%20non-beta%20cells%20in%20response%20to%20beta-cell%20mitogens%20in%20isolated%20human%20islets%20of%20Langerhans.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2011283.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-90643-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-90643-3%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3D3Z68GUVE%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Pronounced%20proliferation%20of%20non-beta%20cells%20in%20response%20to%20beta-cell%20mitogens%20in%20isolated%20human%20islets%20of%20Langerhans%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hasna%22%2C%22lastName%22%3A%22Maachi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julien%22%2C%22lastName%22%3A%22Ghislain%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Tremblay%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vincent%22%2C%22lastName%22%3A%22Poitout%22%7D%5D%2C%22abstractNote%22%3A%22The%20potential%20to%20treat%20diabetes%20by%20increasing%20beta-cell%20mass%20is%20driving%20a%20major%20effort%20to%20identify%20beta-cell%20mitogens.%20Demonstration%20of%20mitogen%20activity%20in%20human%20beta%20cells%20is%20frequently%20performed%20in%20ex%20vivo%20assays.%20However%2C%20reported%20disparities%20in%20the%20efficacy%20of%20beta-cell%20mitogens%20led%20us%20to%20investigate%20the%20sources%20of%20this%20variability.%20We%20studied%2035%20male%20%2823%29%20and%20female%20%2812%29%20human%20islet%20batches%20covering%20a%20range%20of%20donor%20ages%20and%20BMI.%20Islets%20were%20kept%20intact%20or%20dispersed%20into%20single%20cells%20and%20cultured%20in%20the%20presence%20of%20harmine%2C%20glucose%2C%20or%20heparin-binding%20epidermal%20growth%20factor-like%20growth%20factor%20%28HB-EGF%29%2C%20and%20subsequently%20analyzed%20by%20immunohistochemistry%20or%20flow%20cytometry.%20Proliferating%20cells%20were%20identified%20by%20double%20labeling%20with%20EdU%20and%20Ki67%20and%20glucagon%2C%20c-peptide%20or%20Nkx6.1%2C%20and%20cytokeratin-19%20to%20respectively%20label%20alpha%2C%20beta%2C%20and%20ductal%20cells.%20Harmine%20and%20HB-EGF%20stimulated%20human%20beta-cell%20proliferation%2C%20but%20the%20effect%20of%20glucose%20was%20dependent%20on%20the%20assay%20and%20the%20donor.%20Harmine%20potently%20stimulated%20alpha-cell%20proliferation%20and%20both%20harmine%20and%20HB-EGF%20increased%20proliferation%20of%20insulin-%20and%20glucagon-negative%20cells%2C%20including%20cytokeratin%2019-positive%20cells.%20Given%20the%20abundance%20of%20non-beta%20cells%20in%20human%20islet%20preparations%2C%20our%20results%20suggest%20that%20assessment%20of%20beta-cell%20mitogens%20requires%20complementary%20approaches%20and%20rigorous%20identification%20of%20cell%20identity%20using%20multiple%20markers.%22%2C%22date%22%3A%222021-05-28%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-90643-3%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-90643-3%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%223XSKJF5U%22%5D%2C%22dateModified%22%3A%222021-05-28T17%3A44%3A49Z%22%7D%7D%5D%7D
Maachi, H., Ghislain, J., Tremblay, C., & Poitout, V. (2021). Pronounced proliferation of non-beta cells in response to beta-cell mitogens in isolated human islets of Langerhans. Scientific Reports, 11(1), 11283. https://doi.org/10.1038/s41598-021-90643-3 Cite
