Intrinsic Activation of β-Catenin Signaling by CRISPR/Cas9-Mediated Exon Skipping Contributes to Immune Evasion in Hepatocellular Carcinoma

Researchers established human and mouse hepatocellular carcinoma cells with exon 3 skipping of β-catenin, which promoted nuclear translocation and activated the Wnt/β-catenin signaling pathway, by using newly developed multiplex CRISPR/Cas9-based genome engineering system.