A Versatile and Robust Cell Purification System with an RNA-Only Circuit Composed of microRNA-Responsive ON and OFF Switches

By designing RNA-only circuits with miRNA-ON and -OFF switch mRNAs that encoded a lethal ribonuclease, Barnase, and its inhibitor, Barstar, the authors efficiently purified specific cell types, including human iPSCs and differentiated cardiomyocytes, without flow cytometry.