Scientists highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography.
[Frontiers in Bioengineering and Biotechnology]
6445212 {6445212:VMNC3CF9} apa 50 1 155050 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-9c18370166c9d7fa5d0a2f4b0efed421%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22VMNC3CF9%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Tran%20et%20al.%22%2C%22parsedDate%22%3A%222020%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ETran%2C%20R.%2C%20Moraes%2C%20C.%2C%20%26amp%3B%20Hoesli%2C%20C.%20A.%20%282020%29.%20Developmentally-Inspired%20Biomimetic%20Culture%20Models%20to%20Produce%20Functional%20Islet-Like%20Cells%20From%20Pluripotent%20Precursors.%20%3Ci%3EFrontiers%20in%20Bioengineering%20and%20Biotechnology%3C%5C%2Fi%3E%2C%20%3Ci%3E8%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffbioe.2020.583970%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3389%5C%2Ffbioe.2020.583970%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DVMNC3CF9%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Developmentally-Inspired%20Biomimetic%20Culture%20Models%20to%20Produce%20Functional%20Islet-Like%20Cells%20From%20Pluripotent%20Precursors%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Raymond%22%2C%22lastName%22%3A%22Tran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Moraes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Corinne%20A.%22%2C%22lastName%22%3A%22Hoesli%22%7D%5D%2C%22abstractNote%22%3A%22Insulin-producing%20beta%20cells%20sourced%20from%20pluripotent%20stem%20cells%20hold%20great%20potential%20as%20a%20virtually%20unlimited%20cell%20source%20to%20treat%20diabetes.%20Directed%20pancreatic%20differentiation%20protocols%20aim%20to%20mimic%20various%20stimuli%20present%20during%20embryonic%20development%20through%20sequential%20changes%20of%20in%20vitro%20culture%20conditions.%20This%20is%20commonly%20accomplished%20by%20the%20timed%20addition%20of%20soluble%20signaling%20factors%2C%20in%20conjunction%20with%20cell-handling%20steps%20such%20as%20the%20formation%20of%203D%20cell%20aggregates.%20Interestingly%2C%20when%20stem%20cells%20at%20the%20pancreatic%20progenitor%20stage%20are%20transplanted%2C%20they%20form%20functional%20insulin-producing%20cells%2C%20suggesting%20that%20in%20vivo%20microenvironmental%20cues%20promote%20beta%20cell%20specification.%20Among%20these%20cues%2C%20biophysical%20stimuli%20have%20only%20recently%20emerged%20in%20the%20context%20of%20optimizing%20pancreatic%20differentiation%20protocols.%20This%20review%20focuses%20on%20studies%20of%20cell-microenvironment%20interactions%20and%20their%20impact%20on%20differentiating%20pancreatic%20cells%20when%20considering%20cell%20signaling%2C%20cell-cell%20and%20cell-ECM%20interactions.%20We%20highlight%20the%20development%20of%20in%20vitro%20cell%20culture%20models%20that%20allow%20systematic%20studies%20of%20pancreatic%20cell%20mechanobiology%20in%20response%20to%20extracellular%20matrix%20proteins%2C%20biomechanical%20effects%2C%20soluble%20factor%20modulation%20of%20biomechanics%2C%20substrate%20stiffness%2C%20fluid%20flow%20and%20topography.%20Finally%2C%20we%20explore%20how%20these%20new%20mechanical%20insights%20could%20lead%20to%20novel%20pancreatic%20differentiation%20protocols%20that%20improve%20efficiency%2C%20maturity%2C%20and%20throughput.%22%2C%22date%22%3A%222020%22%2C%22language%22%3A%22English%22%2C%22DOI%22%3A%2210.3389%5C%2Ffbioe.2020.583970%22%2C%22ISSN%22%3A%222296-4185%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.frontiersin.org%5C%2Farticles%5C%2F10.3389%5C%2Ffbioe.2020.583970%5C%2Ffull%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-10-09T16%3A42%3A57Z%22%7D%7D%5D%7D
Tran, R., Moraes, C., & Hoesli, C. A. (2020). Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.583970 Cite