Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsCellular deregulation was assessed by measuring glutathione levels, basal calcium and intracellular calcium response under KCl stimulation, as well as the key parameters of proliferation, glial progenitor marker expression and migration.
[Cellular and Molecular Neurobiology]
6445212 {6445212:C8MYKXJK} apa 50 1 156678 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-0963926c69087f0452b6171010d28c90%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22C8MYKXJK%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sowmithra%20et%20al.%22%2C%22parsedDate%22%3A%222020-11-18%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESowmithra%2C%20S.%2C%20Jain%2C%20N.%20K.%2C%20Bhonde%2C%20R.%2C%20%26amp%3B%20Datta%2C%20I.%20%282020%29.%20Recovery%20of%20Human%20Embryonic%20Stem%20Cells-Derived%20Neural%20Progenitors%20Exposed%20to%20Hypoxic-Ischemic-Reperfusion%20Injury%20by%20Indirect%20Exposure%20to%20Wharton%26%23x2019%3Bs%20Jelly%20Mesenchymal%20Stem%20Cells%20Through%20Phosphatidyl-inositol-3-Kinase%20Pathway.%20%3Ci%3ECellular%20and%20Molecular%20Neurobiology%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs10571-020-01007-w%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs10571-020-01007-w%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DC8MYKXJK%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Recovery%20of%20Human%20Embryonic%20Stem%20Cells-Derived%20Neural%20Progenitors%20Exposed%20to%20Hypoxic-Ischemic-Reperfusion%20Injury%20by%20Indirect%20Exposure%20to%20Wharton%5Cu2019s%20Jelly%20Mesenchymal%20Stem%20Cells%20Through%20Phosphatidyl-inositol-3-Kinase%20Pathway%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sowmithra%22%2C%22lastName%22%3A%22Sowmithra%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nishtha%20Kusum%22%2C%22lastName%22%3A%22Jain%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ramesh%22%2C%22lastName%22%3A%22Bhonde%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Indrani%22%2C%22lastName%22%3A%22Datta%22%7D%5D%2C%22abstractNote%22%3A%22Increasing%20evidence%20suggests%20that%20mesenchymal%20stem%20cells%28MSCs%29%20have%20beneficial%20effects%20in%20hypoxic%20ischemic%20reperfusion%20injury%2C%20but%20the%20underlying%20mechanisms%20are%20unclear.%20Here%2C%20we%20first%20examined%20the%20effect%20of%20OGD%20reperfusion%20injury%20on%20the%20vulnerability%20of%20human%20NPs%20derived%20from%20human%20embryonic%20stem%20cells%20%28hESCs%29%20with%20regard%20to%20cell%20survival%20and%20oxidative%20stress.%20Cellular%20deregulation%20was%20assessed%20by%20measuring%20glutathione%20levels%2C%20basal%20calcium%20and%20intracellular%20calcium%20%5BCa2%2B%5Di%20response%20under%20KCl%20stimulation%2C%20as%20well%20as%20the%20key%20parameters%20of%20proliferation%2C%20glial%20progenitor%20marker%20expression%20and%20migration.%20Next%2C%20the%20influence%20of%20WJ-MSCs%20in%20recovering%20these%20parameters%20was%20evaluated%2C%20and%20the%20role%20of%20Phosphatidyl-inositol-3-Kinase%28PI3K%29%20pathway%20in%20actuating%20the%20protective%20effect%20was%20assessed.%20OGD%20reperfusion%20injury%20induced%20significant%20increases%20in%20cell%20death%2C%20ROS%20generation%2C%20oxidative%20stress%20susceptibility%20and%20decreased%20glutathione%20levels%20in%20NPs%2C%20accompanied%20by%20rises%20in%20basal%20%5BCa2%2B%5Di%2C%20KCl-induced%20%5BCa2%2B%5Di%2C%20expression%20of%20K%2B%20leak%20channel%28TASK1%29%2C%20and%20declines%20in%20proliferation%2C%20migration%20potential%20and%20glial%20progenitor%20population.%20The%20introduction%20of%20WJ-MSCs%28after%202%5Cu00a0h%20of%20reperfusion%29%20through%20a%20non-contact%20method%20brought%20about%20significant%20improvement%20in%20all%20these%20cellular%20parameters%20as%20observed%20after%2024hrs%2C%20and%20the%20PI3K%20pathway%20played%20an%20important%20role%20in%20the%20neuroprotection%20process.%20Presence%20of%20WJ-MSCs%20increased%20the%20expression%20of%20survival%20signals%20like%20phosphorylated%20Akt%5C%2FAkt%20and%20PI3K%20in%20the%20OGD-reperfused%20NPs.%20Our%20data%20clearly%20demonstrate%20for%20the%20first%20time%20that%20soluble%20factors%20from%20WJ-MSCs%20can%20not%20only%20ameliorate%20survival%2C%20proliferation%2C%20migration%20and%20glial%20progenitor%20expression%20of%20OGD-reperfused%20NPs%2C%20but%20also%20regulate%20their%20intracellular%20Ca2%2B%20response%20to%20KCl%20stimulation%20and%20expression%20of%20TASK1%20through%20the%20PI3K%20pathway.%22%2C%22date%22%3A%222020-11-18%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1007%5C%2Fs10571-020-01007-w%22%2C%22ISSN%22%3A%221573-6830%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs10571-020-01007-w%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-11-26T00%3A18%3A37Z%22%7D%7D%5D%7D
Sowmithra, S., Jain, N. K., Bhonde, R., & Datta, I. (2020). Recovery of Human Embryonic Stem Cells-Derived Neural Progenitors Exposed to Hypoxic-Ischemic-Reperfusion Injury by Indirect Exposure to Wharton’s Jelly Mesenchymal Stem Cells Through Phosphatidyl-inositol-3-Kinase Pathway. Cellular and Molecular Neurobiology. https://doi.org/10.1007/s10571-020-01007-w Cite
