Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsExosome marker protein levels were increased in the exosomes derived from human umbilical cord MSCs (hUMSC-Ex) compared with those in hUMSC suspension, indicating that exosomes were successfully isolated from hUMSCs.
[Acta Biochimica et Biophysica Sinica]
6445218 PPNK3LMQ items 1 apa 0 default asc 1 164496 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-c3b5fc8760085709f2ebad66b429eebf%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22PPNK3LMQ%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Gao%20et%20al.%22%2C%22parsedDate%22%3A%222021-07-13%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGao%2C%20H.%2C%20Yu%2C%20Z.%2C%20Li%2C%20Y.%2C%20%26amp%3B%20Wang%2C%20X.%20%282021%29.%20miR-100-5p%20in%20human%20umbilical%20cord%20mesenchymal%20stem%20cell%26%23x2013%3Bderived%20exosomes%20mediates%20eosinophilic%20inflammation%20to%20alleviate%20atherosclerosis%20via%20the%20FZD5%5C%2FWnt%5C%2F%26%23x3B2%3B-catenin%20pathway.%20%3Ci%3EActa%20Biochimica%20et%20Biophysica%20Sinica%3C%5C%2Fi%3E%2C%20%3Ci%3Egmab093%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fabbs%5C%2Fgmab093%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fabbs%5C%2Fgmab093%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DPPNK3LMQ%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22miR-100-5p%20in%20human%20umbilical%20cord%20mesenchymal%20stem%20cell%5Cu2013derived%20exosomes%20mediates%20eosinophilic%20inflammation%20to%20alleviate%20atherosclerosis%20via%20the%20FZD5%5C%2FWnt%5C%2F%5Cu03b2-catenin%20pathway%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Heng%22%2C%22lastName%22%3A%22Gao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhanbiao%22%2C%22lastName%22%3A%22Yu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yuanyuan%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xue%22%2C%22lastName%22%3A%22Wang%22%7D%5D%2C%22abstractNote%22%3A%22Exosomes%20derived%20from%20human%20umbilical%20cord%20mesenchymal%20stem%20cells%20%28hUMSC-Ex%29%20play%20important%20roles%20in%20immune%20and%20inflammation%20diseases.%20However%2C%20the%20role%20of%20hUMSC-Ex%20in%20atherosclerosis%20has%20not%20been%20elucidated.%20In%20this%20study%2C%20the%20isolated%20exosomes%20were%20identified%20by%20transmission%20electron%20microscopy%20and%20nanoparticle%20tracking%20analysis.%20Exosome%20marker%20protein%20levels%20were%20increased%20in%20the%20hUMSC-Ex%20compared%20with%20those%20in%20hUMSC%20suspension%2C%20indicating%20that%20exosomes%20were%20successfully%20isolated%20from%20hUMSCs.%20Furthermore%2C%20eosinophils%20were%20treated%20with%20oxidized%20low-density%20lipoprotein%20%28ox-LDL%29%20to%20construct%20inflammation%20model%20and%20then%20incubated%20with%20hUMSC-Ex%20derived%20from%20hUMSCs%20which%20were%20transfected%20with%20miR-100-5p%20mimic%20or%20miR-100-5p%20inhibitor.%20We%20found%20that%20hUMSC-Ex%20increased%20miR-100-5p%20expression%2C%20inhibited%20cell%20migration%2C%20promoted%20cell%20apoptosis%2C%20and%20reduced%20inflammatory%20cytokine%20levels%20in%20ox-LDL-treated%20eosinophils%2C%20and%20miR-100-5p%20overexpression%20in%20hUMSCs%20enhanced%20these%20effects%2C%20while%20miR-100-5p%20inhibition%20reversed%20these%20effects.%20Moreover%2C%20frizzled%205%20%28FZD5%29%20was%20a%20target%20gene%20of%20miR-100-5p.%20FZD5%20overexpression%20reversed%20the%20inhibitory%20effects%20of%20hUMSC-Ex-miR-100-5p%20on%20cell%20progression%20and%20inflammation%20in%20eosinophils.%20Additionally%2C%20hUMSC-Ex-miR-100-5p%20decreased%20the%20expression%20of%20cyclin%20D1%20and%20%5Cu03b2-catenin%20proteins.%20Wnt%5C%2F%5Cu03b2-catenin%20pathway%20activator%20BML-284%20effectively%20reversed%20the%20effects%20of%20hUMSC-Ex-miR-100-5p%20on%20cell%20progression%20and%20inflammation%20in%20eosinophils.%20ApoE%5Cu2212%5C%2F%5Cu2212%20mice%20were%20fed%20with%20high-fat%20diet%20to%20construct%20an%20atherosclerosis%20mice%20model%2C%20and%20hUMSC-Ex%20was%20injected%20into%20mice.%20HUMSC-Ex%20reduced%20atherosclerotic%20plaque%20area%20and%20inflammation%20response%20in%20atherosclerosis%20mice.%20This%20study%20demonstrates%20that%20hUMSC-Ex-miR-100-5p%20inhibits%20cell%20progression%20and%20inflammatory%20response%20in%20eosinophils%20via%20the%20FZD5%5C%2FWnt%5C%2F%5Cu03b2-catenin%20pathway%2C%20thereby%20alleviating%20atherosclerosis%20progression.%22%2C%22date%22%3A%22July%2013%2C%202021%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fabbs%5C%2Fgmab093%22%2C%22ISSN%22%3A%221745-7270%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fabbs%5C%2Fgmab093%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-07-13T18%3A30%3A20Z%22%7D%7D%5D%7D
Gao, H., Yu, Z., Li, Y., & Wang, X. (2021). miR-100-5p in human umbilical cord mesenchymal stem cell–derived exosomes mediates eosinophilic inflammation to alleviate atherosclerosis via the FZD5/Wnt/β-catenin pathway. Acta Biochimica et Biophysica Sinica, gmab093. https://doi.org/10.1093/abbs/gmab093 Cite
