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western blot analysis

Interleukin-27 Promotes Autophagy in Human Serum-Induced Primary Macrophages via an mTOR- and LC3-Independent Pathway

[Scientific Reports] Researchers investigated the impact of IL-27 in both autophagy induction and HIV-1 infection in macrophages.

Histone H2B Ubiquitination Mediated Chromatin Relaxation Is Essential for the Induction of Somatic Cell Reprogramming

[Cell Proliferation] RNA-seq, quantitative RT-PCR and western blot analysis were applied to study the role of RNF20 and H2B ubiquitination during mouse somatic cell reprogramming.

M2 Macrophages, but Not M1 Macrophages, Support Megakaryopoiesis by Upregulating PI3K-AKT Pathway Activity

[Signal Transduction and Targeted Therapy] Aberrant bone marrow-M1/M2 MФ polarization, characterized by increased M1 MФs and decreased M2 MФs and accompanied by impaired megakaryopoiesis-supporting abilities, was found in patients with thrombocytopenia post-allotransplant.

Bile Acid-Induced Tissue Factor Activity in Hepatocytes Correlates with Activation of Farnesoid X Receptor

[Laboratory Investigation] Researchers investigated the effects of bile acids on tissue factor activity and thrombin generation in hepatocytes and correlated these effects with activation of farnesoid X receptor-dependent signaling and apoptosis.

miR-141-5p Suppresses Vascular Smooth Muscle Cell Inflammation, Proliferation, and Migration via Inhibiting the HMGB1/NF-κB Pathway

[Journal of Biochemical and Molecular Toxicology] Using human vascular smooth muscle cells (VSMCs) treated by oxidized low-density lipoprotein to establish an atherosclerosis cell model, researchers explored the functional roles and molecular mechanisms of miR-141-5p in VSMCs dysfunction.

Isorhapontigenin Ameliorates Cerebral Ischemia/Reperfusion Injury via Modulating Kinase Cε/Nrf2/HO-1 Signaling Pathway

[Brain and Behavior] Focal cerebral ischemia-reperfusion injury model and primary cortical neurons were established an oxygen-glucose deprivation injury model. Neuronal cell viability was determined by MTT assay. Western blot analysis was determined for protein expression.

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