By sequencing the exomes of the microdissected clusters of lymphoma cells from the epidermis and the dermis, scientists found that those microenvironments comprised different malignant clonotypes.
[Scientific Reports]
6807162 {6807162:3MGH4Q6D} apa 50 1 154498 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-d03f61fa6109bab5d6e631904348f718%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%223MGH4Q6D%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Iyer%20et%20al.%22%2C%22parsedDate%22%3A%222020-09-23%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EIyer%2C%20A.%2C%20Hennessey%2C%20D.%2C%20O%26%23x2019%3BKeefe%2C%20S.%2C%20Patterson%2C%20J.%2C%20Wang%2C%20W.%2C%20Wong%2C%20G.%20K.-S.%2C%20%26amp%3B%20Gniadecki%2C%20R.%20%282020%29.%20Independent%20evolution%20of%20cutaneous%20lymphoma%20subclones%20in%20different%20microenvironments%20of%20the%20skin.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E%281%29%2C%2015483.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-020-72459-9%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-020-72459-9%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3D3MGH4Q6D%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Independent%20evolution%20of%20cutaneous%20lymphoma%20subclones%20in%20different%20microenvironments%20of%20the%20skin%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aishwarya%22%2C%22lastName%22%3A%22Iyer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dylan%22%2C%22lastName%22%3A%22Hennessey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sandra%22%2C%22lastName%22%3A%22O%5Cu2019Keefe%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jordan%22%2C%22lastName%22%3A%22Patterson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Weiwei%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gane%20Ka-Shu%22%2C%22lastName%22%3A%22Wong%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%22%2C%22lastName%22%3A%22Gniadecki%22%7D%5D%2C%22abstractNote%22%3A%22Mycosis%20fungoides%20%28MF%29%20is%20the%20most%20common%20cutaneous%20T-cell%20lymphoma.%20Lesions%20of%20MF%20are%20formed%20by%20hematogenous%20seeding%20the%20skin%20with%20polyclonal%20%28clonotypically%20diverse%29%20neoplastic%20T-cells%20which%20accumulate%20numerous%20mutations%20and%20display%20a%20high%20degree%20of%20mutational%2C%20intratumoral%20heterogeneity%20%28ITH%29.%20A%20characteristic%20but%20poorly%20studied%20feature%20of%20MF%20is%20epidermotropism%2C%20the%20tendency%20to%20infiltrate%20skin%20epithelial%20layer%20%28epidermis%29%20in%20addition%20to%20the%20vascularized%20dermis.%20By%20sequencing%20the%20exomes%20of%20the%20microdissected%20clusters%20of%20lymphoma%20cells%20from%20the%20epidermis%20and%20the%20dermis%2C%20we%20found%20that%20those%20microenvironments%20comprised%20different%20malignant%20clonotypes.%20Subclonal%20structure%20witnessed%20the%20independent%20mutational%20evolution%20in%20the%20epidermis%20and%20dermis.%20Thus%2C%20the%20epidermal%20involvement%20in%20MF%20could%20not%20be%20explained%20by%20gradual%20infiltration%20from%20the%20dermis%20but%20was%20caused%20by%20a%20separate%20seeding%20process%20followed%20by%20a%20quasi-neutral%2C%20branched%20evolution.%20In%20conclusion%2C%20tissue%20microenvironments%20shape%20the%20subclonal%20architecture%20in%20MF%20leading%20to%20%5Cu201cecological%20heterogeneity%5Cu201d%20which%20contributes%20to%20the%20total%20ITH.%20Since%20ITH%20adversely%20affects%20cancer%20prognosis%2C%20targeting%20the%20microenvironment%20may%20present%20therapeutic%20opportunities%20in%20MF%20and%20other%20cancers.%22%2C%22date%22%3A%222020-09-23%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-020-72459-9%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-020-72459-9%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-09-23T22%3A56%3A12Z%22%7D%7D%5D%7D
Iyer, A., Hennessey, D., O’Keefe, S., Patterson, J., Wang, W., Wong, G. K.-S., & Gniadecki, R. (2020). Independent evolution of cutaneous lymphoma subclones in different microenvironments of the skin. Scientific Reports, 10(1), 15483. https://doi.org/10.1038/s41598-020-72459-9 Cite