Scientists present a step-by-step methodology to generate human minibrain nurseries and novel strategies to subsequently label projection neurons, perform immunohistochemistry and 3D imaging of the minibrains at large multiplexable scales.
[Frontiers in Bioengineering and Biotechnology]
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Govindan, S., Batti, L., Osterop, S. F., Stoppini, L., & Roux, A. (2021). Mass Generation, Neuron Labeling, and 3D Imaging of Minibrains. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.582650 Cite