Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsResearchers show CREB phosphorylation was required for smooth muscle cell proliferation in response to platelet-derived growth factor (PDGF). This phenotypic change required CBP and was mediated by Cyclin D1 and p27kip as a result of changes in FoxO1 activity.
[Experimental Cell Research]
6606345 {6606345:JIJESR3D} apa 50 1 161587 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-c119b1e058def088e617fc4ab1c027c9%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22JIJESR3D%22%2C%22library%22%3A%7B%22id%22%3A6606345%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Perrault%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-22%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPerrault%2C%20R.%2C%20Molnar%2C%20P.%2C%20Poole%2C%20J.%2C%20%26amp%3B%20Zahradka%2C%20P.%20%282021%29.%20PDGF-mediated%20activation%20of%20CREB%20in%20vascular%20smooth%20muscle%20cells%20alters%20cell%20cycling%20via%20Rb%2C%20FoxO1%20and%20p27kip1.%20%3Ci%3EExperimental%20Cell%20Research%3C%5C%2Fi%3E%2C%20112612.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.yexcr.2021.112612%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.yexcr.2021.112612%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6606345%26amp%3Bitem_key%3DJIJESR3D%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22PDGF-mediated%20activation%20of%20CREB%20in%20vascular%20smooth%20muscle%20cells%20alters%20cell%20cycling%20via%20Rb%2C%20FoxO1%20and%20p27kip1%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Raissa%22%2C%22lastName%22%3A%22Perrault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Molnar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jenna%22%2C%22lastName%22%3A%22Poole%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peter%22%2C%22lastName%22%3A%22Zahradka%22%7D%5D%2C%22abstractNote%22%3A%22Introduction%20%26%20Aim%5CnThe%20vascular%20response%20to%20injury%20leads%20to%20the%20secretion%20of%20several%20factors%2C%20including%20platelet-derived%20growth%20factor%20%28PDGF%29.%20PDGF%20stimulates%20smooth%20muscle%20cell%20%28SMC%29%20conversion%20to%20the%20synthetic%20phenotype%2C%20thereby%20enhancing%20proliferation%20and%20migration%2C%20and%20contributing%20to%20neointimal%20hyperplasia.%20Likewise%2C%20the%20transcription%20factor%20CREB%20has%20been%20shown%20to%20mediate%20SMC%20proliferation%20in%20response%20to%20various%20mitogens.%20We%20therefore%20investigated%20the%20contribution%20of%20CREB%20to%20PDGF-dependent%20proliferation%20of%20SMCs%20with%20the%20intention%20of%20identifying%20signaling%20pathways%20involved%20both%20up%20and%20downstream%20of%20CREB%20activation.%5CnMethods%20%26%20Results%5CnTreatments%20were%20performed%20on%20vascular%20SMCs%20from%20a%20porcine%20coronary%20artery%20explant%20model.%20The%20role%20of%20CREB%20was%20examined%20via%20adenoviral%20expression%20of%20a%20dominant-negative%20CREB%20mutant%20%28kCREB%29%20and%20involvement%20of%20the%20p27kip1%20pathway%20was%20determined%20using%20a%20constitutively%20expressing%20p27kip1%20mutant.%20PDGF%20stimulated%20transient%20CREB%20phosphorylation%20on%20Ser-133%20via%20ERK1%5C%2F2-%2C%20PI3-kinase-%20and%20Src-dependent%20pathways.%20Expression%20of%20kCREB%20decreased%20PDGF-dependent%20cell%20proliferation.%20PCNA%20expression%20and%20Rb%20phosphorylation%20were%20also%20inhibited%20by%20kCREB.%20These%20cell%20cycle%20proteins%20are%20controlled%20via%20p27kip1%20expression%20in%20response%20to%20CREB-dependent%20post-translational%20modification%20of%20FoxO1.%20kCREB%20had%20no%20effect%20on%20Cyclin%20D1%20expression%2C%20but%20did%20prevent%20PDGF-induced%20Cyclin%20D1%20nuclear%20translocation.%20An%20interaction%20inhibitor%20of%20CBP%20confirms%20that%20Cyclin%20D1%20is%20downstream%20of%20PDGF%20and%20CREB.%5CnConclusion%5CnCREB%20phosphorylation%20is%20required%20for%20SMC%20proliferation%20in%20response%20to%20PDGF.%20This%20phenotypic%20change%20requires%20CBP%20and%20is%20mediated%20by%20Cyclin%20D1%20and%20p27kip%20as%20a%20result%20of%20changes%20in%20FoxO1%20activity.%22%2C%22date%22%3A%22April%2022%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.yexcr.2021.112612%22%2C%22ISSN%22%3A%220014-4827%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0014482721001440%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-26T22%3A27%3A00Z%22%7D%7D%5D%7D
Perrault, R., Molnar, P., Poole, J., & Zahradka, P. (2021). PDGF-mediated activation of CREB in vascular smooth muscle cells alters cell cycling via Rb, FoxO1 and p27kip1. Experimental Cell Research, 112612. https://doi.org/10.1016/j.yexcr.2021.112612 Cite
